caa a22 4500 606236376 CHVBK 20210128101247.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s11010-014-2269-y doi (NATIONALLICENCE)springer-10.1007/s11010-014-2269-y RETRACTED ARTICLE: Identification of genes associated with the differentiation potential of adipose-derived stem cells to osteocytes or myocytes [Elektronische Daten] [Yizhong Ren, Changxu Han, Jingjuan Wang, Yanbo Jia, Lingyue Kong, Tu Eerdun, Lishuan Wu, Dianming Jiang] Adipose-derived stem cells (ADSCs) have been considered as the optimal cells for regenerative medicine because ADSCs have the potential of multi-directional differentiation. To study the mechanisms of ADSCs differentiation, we analyzed microarray of GSE37329. GSE37329 was downloaded from Gene Expression Omnibus including 3 ADSCs, 2 ADSCs-derived osteocytes, and 2 ADSCs-derived myocytes samples. The differentially expressed genes (DEGs) were screened using limma package. Their underlying functions were predicted by gene ontology and pathway enrichment analyses. Besides, the interaction relationships of the proteins encoded by DEGs were obtained from STRING database, and protein-protein interaction (PPI) network was constructed using Cytoscape. Furthermore, modules analysis of PPI network was performed using MCODE in Cytoscape. We screened 662 and 484 DEG separately for the ADSCs-derived osteocytes and myocytes compared with ADSCs. There were 205 common up-regulated and 128 common down-regulated DEGs between the two groups. Function enrichment indicated that these common DEGs, especially, VEGFA, FGF2, and EGR1 may be related to cell differentiation. PPI network for common DEGs also suggested that VEGFA (degree=29), FGF2 (degree=17), and EGR1 (degree=12) might be more important because they had higher connectivity degrees, and they might be involved in cell differentiation by interacting with other genes in module A (e.g., EGR1-NGF and EGR1-LEP), and B (e.g., VEGFA-PDGFD). Additionally, the IGF1 and BTG1 may be, respectively, specific for osteocytes and myocytes differentiation. VEGFA, PDGFD, FGF2, EGR1, NGF, LEP, IGF1, and BTG1 might serve as target genes in regulating ADSCs differentiation. Springer Science+Business Media New York, 2014 Adipose-derived stem cells nationallicence Differentially expressed genes nationallicence Protein-protein interaction network nationallicence Module analysis nationallicence Ren Yizhong Department of Orthopaedics, The First Hospital of Chongqing Medical University, No. 1 Youyi Road, Yuzhong District, 400016, Chongqing City, China aut Han Changxu Department of Arthroscopy and Sports Medicine, The Second Affiliated Hospital of Inner Mongolia Medical University, No. 1 Yingfang Street, Hyumin District, 010055, Huhehot, Inner Mongolia, China aut Wang Jingjuan Department of Arthroscopy and Sports Medicine, The Second Affiliated Hospital of Inner Mongolia Medical University, No. 1 Yingfang Street, Hyumin District, 010055, Huhehot, Inner Mongolia, China aut Jia Yanbo Department of Arthroscopy and Sports Medicine, The Second Affiliated Hospital of Inner Mongolia Medical University, No. 1 Yingfang Street, Hyumin District, 010055, Huhehot, Inner Mongolia, China aut Kong Lingyue Department of Arthroscopy and Sports Medicine, The Second Affiliated Hospital of Inner Mongolia Medical University, No. 1 Yingfang Street, Hyumin District, 010055, Huhehot, Inner Mongolia, China aut Eerdun Tu Department of Arthroscopy and Sports Medicine, The Second Affiliated Hospital of Inner Mongolia Medical University, No. 1 Yingfang Street, Hyumin District, 010055, Huhehot, Inner Mongolia, China aut Wu Lishuan Department of Anesthesiology, The Second Hospital of Inner Mongolia Medical University, No. 1 Yingfang Street, Hyumin District, 010055, Huhehot, Inner Mongolia, China aut Jiang Dianming Department of Orthopaedics, The First Hospital of Chongqing Medical University, No. 1 Youyi Road, Yuzhong District, 400016, Chongqing City, China aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 400/1-2(2015-02-01), 135-144 0300-8177 400:1-2<135 2015 400 11010 https://doi.org/10.1007/s11010-014-2269-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-014-2269-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ren Yizhong Department of Orthopaedics, The First Hospital of Chongqing Medical University, No. 1 Youyi Road, Yuzhong District, 400016, Chongqing City, China aut NATIONALLICENCE 700 1- Han Changxu Department of Arthroscopy and Sports Medicine, The Second Affiliated Hospital of Inner Mongolia Medical University, No. 1 Yingfang Street, Hyumin District, 010055, Huhehot, Inner Mongolia, China aut NATIONALLICENCE 700 1- Wang Jingjuan Department of Arthroscopy and Sports Medicine, The Second Affiliated Hospital of Inner Mongolia Medical University, No. 1 Yingfang Street, Hyumin District, 010055, Huhehot, Inner Mongolia, China aut NATIONALLICENCE 700 1- Jia Yanbo Department of Arthroscopy and Sports Medicine, The Second Affiliated Hospital of Inner Mongolia Medical University, No. 1 Yingfang Street, Hyumin District, 010055, Huhehot, Inner Mongolia, China aut NATIONALLICENCE 700 1- Kong Lingyue Department of Arthroscopy and Sports Medicine, The Second Affiliated Hospital of Inner Mongolia Medical University, No. 1 Yingfang Street, Hyumin District, 010055, Huhehot, Inner Mongolia, China aut NATIONALLICENCE 700 1- Eerdun Tu Department of Arthroscopy and Sports Medicine, The Second Affiliated Hospital of Inner Mongolia Medical University, No. 1 Yingfang Street, Hyumin District, 010055, Huhehot, Inner Mongolia, China aut NATIONALLICENCE 700 1- Wu Lishuan Department of Anesthesiology, The Second Hospital of Inner Mongolia Medical University, No. 1 Yingfang Street, Hyumin District, 010055, Huhehot, Inner Mongolia, China aut NATIONALLICENCE 700 1- Jiang Dianming Department of Orthopaedics, The First Hospital of Chongqing Medical University, No. 1 Youyi Road, Yuzhong District, 400016, Chongqing City, China aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 400/1-2(2015-02-01), 135-144 0300-8177 400:1-2<135 2015 400 11010