caa a22 4500 606236392 CHVBK 20210128101247.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s11010-014-2261-6 doi (NATIONALLICENCE)springer-10.1007/s11010-014-2261-6 Curcumin and quercetin trigger apoptosis during benzo(a)pyrene-induced lung carcinogenesis [Elektronische Daten] [Praveen Nair, Anshoo Malhotra, Devinder Dhawan] The aim of the present study was to assess the cumulative effects of curcumin and quercetin in inducing apoptosis during benzo(a)pyrene (BP) (100mg/Kg body weight)-induced lung carcinogenesis in mice. BP treatment resulted in a significant increase in the protein expression of Bcl-2 whereas expression of Bax was significantly decreased. Further, BP treatment brought about a significant decrease in the activities of caspase 3, caspase 9 as well as the number of apoptotic cells. Interestingly, separate as well as combined supplementation of curcumin (60mg/kg body weight) and quercetin (40mg/kg body weight) to BP-treated animals resulted in a significant decrease in the protein expression of Bcl-2 but caused a significant increase in the protein expression of Bax along with a noticeable improvement in the number of apoptotic cells. Also, supplementation with curcumin and quercetin separately to BP-treated mice brought a significant improvement in the enzyme activities of caspase 9 as well as caspase 3 but the improvement was more pronounced following combined treatment. Therefore, curcumin and quercetin, if given in combination shall exhibit enhanced chemopreventive potential against development of lung carcinogenesis by stimulating the apoptotic machinery. Springer Science+Business Media New York, 2014 Lung carcinogenesis nationallicence Apoptosis nationallicence Curcumin nationallicence Quercetin nationallicence Nair Praveen Department of Biomechanics, Sports Authority of India, 560056, Bangalore, India aut Malhotra Anshoo Department of Biophysics, Post Graduate Institute of Medical Education and Research, 160014, Chandigarh, India aut Dhawan Devinder Department of Biophysics, Panjab University, 160014, Chandigarh, India aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 400/1-2(2015-02-01), 51-56 0300-8177 400:1-2<51 2015 400 11010 https://doi.org/10.1007/s11010-014-2261-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-014-2261-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nair Praveen Department of Biomechanics, Sports Authority of India, 560056, Bangalore, India aut NATIONALLICENCE 700 1- Malhotra Anshoo Department of Biophysics, Post Graduate Institute of Medical Education and Research, 160014, Chandigarh, India aut NATIONALLICENCE 700 1- Dhawan Devinder Department of Biophysics, Panjab University, 160014, Chandigarh, India aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 400/1-2(2015-02-01), 51-56 0300-8177 400:1-2<51 2015 400 11010