caa a22 4500 606236406 CHVBK 20210128101247.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s11010-014-2263-4 doi (NATIONALLICENCE)springer-10.1007/s11010-014-2263-4 Circulating levels of apelin, glucagon-like peptide and visfatin in hypercholesterolemic-hyperhomocysteinemic guinea-pigs: their relation with NO metabolism [Elektronische Daten] [Zeynep Kusku-Kiraz, Sema Genc, Seldag Bekpinar, Yesim Unlucerci, Vakur Olgac, Mujdat Uysal, Figen Gurdol] The aim of this study was to determine the levels of regulatory peptides apelin, glucagon-like peptide (GLP-1) and visfatin in hypercholesterolemic and hyperhomocysteinemic state and to examine their relation with nitric oxide (NO) metabolism. 32 Male guinea pigs were divided into four groups and each group was fed as follows: (a) commercial chow, (b) cholesterol (chol)-rich diet, (c) methionine (meth)-rich diet, and (d) chol + meth-rich diet. Blood samples were drawn at the end of 10weeks, and abdominal aorta was dissected for histopathological examination. Serum insulin, GLP-1, apelin, visfatin, and nitrotyrosine concentrations were measured by the manufacturer's kits based on ELISA; asymmetric dimethylarginine (ADMA) and arginine levels were measured by the high performance liquid chromatography. Homocysteine level was measured by the chemiluminescence immunoassay; glucose, total chol and triglyceride levels were measured by the autoanalyzer. The microscopic examination of aorta indicated varying degrees of vascular disturbance in chol- and chol+meth-fed groups. High levels of chol and homocysteine, accompanied with significantly low levels of apelin and GLP-1 were detected in the plasma. Visfatin, ADMA, and nitrotyrosine levels both in chol- and chol+meth-fed groups were significantly higher than those in control animals, whereas arginine and arginine/ADMA ratio were lower. This study indicated that circulating levels of apelin, GLP-1, and visfatin are markedly altered during the development of atherosclerotic changes in close association with chol, homocysteine, NO, and ADMA levels. The measurements of these peptides in serum may help for the diagnosis and follow-up of vascular dysfunction. Springer Science+Business Media New York, 2014 Apelin nationallicence Visfatin nationallicence Glucagon-like peptide nationallicence Hypercholesterolemia nationallicence Nitric oxide nationallicence Hyperhomocysteinemia nationallicence Asymmetric dimethylarginine nationallicence Kusku-Kiraz Zeynep Department of Biochemistry, Istanbul Faculty of Medicine, Istanbul University, Capa, 34093, Istanbul, Turkey aut Genc Sema Department of Biochemistry, Istanbul Faculty of Medicine, Istanbul University, Capa, 34093, Istanbul, Turkey aut Bekpinar Seldag Department of Biochemistry, Istanbul Faculty of Medicine, Istanbul University, Capa, 34093, Istanbul, Turkey aut Unlucerci Yesim Department of Biochemistry, Istanbul Faculty of Medicine, Istanbul University, Capa, 34093, Istanbul, Turkey aut Olgac Vakur Department of Pathology, Institute of Oncology, Istanbul University, Capa, 34093, Istanbul, Turkey aut Uysal Mujdat Department of Biochemistry, Istanbul Faculty of Medicine, Istanbul University, Capa, 34093, Istanbul, Turkey aut Gurdol Figen Department of Biochemistry, Istanbul Faculty of Medicine, Istanbul University, Capa, 34093, Istanbul, Turkey aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 400/1-2(2015-02-01), 69-75 0300-8177 400:1-2<69 2015 400 11010 https://doi.org/10.1007/s11010-014-2263-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-014-2263-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kusku-Kiraz Zeynep Department of Biochemistry, Istanbul Faculty of Medicine, Istanbul University, Capa, 34093, Istanbul, Turkey aut NATIONALLICENCE 700 1- Genc Sema Department of Biochemistry, Istanbul Faculty of Medicine, Istanbul University, Capa, 34093, Istanbul, Turkey aut NATIONALLICENCE 700 1- Bekpinar Seldag Department of Biochemistry, Istanbul Faculty of Medicine, Istanbul University, Capa, 34093, Istanbul, Turkey aut NATIONALLICENCE 700 1- Unlucerci Yesim Department of Biochemistry, Istanbul Faculty of Medicine, Istanbul University, Capa, 34093, Istanbul, Turkey aut NATIONALLICENCE 700 1- Olgac Vakur Department of Pathology, Institute of Oncology, Istanbul University, Capa, 34093, Istanbul, Turkey aut NATIONALLICENCE 700 1- Uysal Mujdat Department of Biochemistry, Istanbul Faculty of Medicine, Istanbul University, Capa, 34093, Istanbul, Turkey aut NATIONALLICENCE 700 1- Gurdol Figen Department of Biochemistry, Istanbul Faculty of Medicine, Istanbul University, Capa, 34093, Istanbul, Turkey aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 400/1-2(2015-02-01), 69-75 0300-8177 400:1-2<69 2015 400 11010