caa a22 4500 606236430 CHVBK 20210128101247.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s11010-014-2285-y doi (NATIONALLICENCE)springer-10.1007/s11010-014-2285-y CXCL13-CXCR5 axis promotes the growth and invasion of colon cancer cells via PI3K/AKT pathway [Elektronische Daten] [Zhenyu Zhu, Xukui Zhang, Hongliang Guo, Ling Fu, Ganlin Pan, Yinggang Sun] CXCL13, an inflammatory factor in the microenvironment, plays a vital role in the progression of inflammatory diseases and tumors. CXCL13 and its receptor CXCR5 have been reported to be associated with poor prognosis of advanced colon cancer. However, the molecular mechanisms of CXCL13-CXCR5 axis in colon cancer remain elusive. The aim of this study was to investigate the role of CXCR5-CXCL13 axis in the growth and invasion of colon cancer cells. Our results showed that CXCL13 promoted the growth, migration, and matrigel invasion of colon cancer cells. Furthermore, CXCL13 increased the expression and secretion of MMP-13, and stimulated the activation of PI3K/AKT pathway. After knockdown of CXCR5 by siRNA, the biological functions of colon cancer cells regulated by CXCL13 were significantly inhibited. In addition, inhibition of PI3K/AKT pathway by specific inhibitor LY294002 suppressed the CXCL13-mediated growth, migration, and invasion of colon cancer cells. Together, our findings suggest that CXCL13-CXCR5 axis promotes the growth, migration, and invasion of colon cancer cells, probably via PI3K/AKT pathway. Thus, CXCL13 may be a useful biomarker for the detection and treatment of colon cancer. Springer Science+Business Media New York, 2014 CXCL13 nationallicence CXCR5 nationallicence Growth nationallicence Invasion nationallicence Colon cancer nationallicence PI3K/AKT pathway nationallicence Zhu Zhenyu Surgiaca Ward 4 (Colorectum & Intestinal Surgery), Shandong Tumor Hospital, 250117, Jinan, Shandong, China aut Zhang Xukui Department of General Surgery, Jinan Military General Hospital, 25 Shifan Road, 250031, Jinan, Shandong, China aut Guo Hongliang Surgiaca Ward 4 (Colorectum & Intestinal Surgery), Shandong Tumor Hospital, 250117, Jinan, Shandong, China aut Fu Ling Department of Oncology, Affiliated Hospital of Weifang Medical University, 261031, Weifang, Shandong, China aut Pan Ganlin Tianqiao Hospital, 250031, Jinan, Shandong, China aut Sun Yinggang Department of General Surgery, Jinan Military General Hospital, 25 Shifan Road, 250031, Jinan, Shandong, China aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 400/1-2(2015-02-01), 287-295 0300-8177 400:1-2<287 2015 400 11010 https://doi.org/10.1007/s11010-014-2285-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-014-2285-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhu Zhenyu Surgiaca Ward 4 (Colorectum & Intestinal Surgery), Shandong Tumor Hospital, 250117, Jinan, Shandong, China aut NATIONALLICENCE 700 1- Zhang Xukui Department of General Surgery, Jinan Military General Hospital, 25 Shifan Road, 250031, Jinan, Shandong, China aut NATIONALLICENCE 700 1- Guo Hongliang Surgiaca Ward 4 (Colorectum & Intestinal Surgery), Shandong Tumor Hospital, 250117, Jinan, Shandong, China aut NATIONALLICENCE 700 1- Fu Ling Department of Oncology, Affiliated Hospital of Weifang Medical University, 261031, Weifang, Shandong, China aut NATIONALLICENCE 700 1- Pan Ganlin Tianqiao Hospital, 250031, Jinan, Shandong, China aut NATIONALLICENCE 700 1- Sun Yinggang Department of General Surgery, Jinan Military General Hospital, 25 Shifan Road, 250031, Jinan, Shandong, China aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 400/1-2(2015-02-01), 287-295 0300-8177 400:1-2<287 2015 400 11010