caa a22 4500 606236449 CHVBK 20210128101247.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s11010-014-2266-1 doi (NATIONALLICENCE)springer-10.1007/s11010-014-2266-1 Studies on mitogen-activated protein kinase signaling pathway in the alveolar macrophages of chronic bronchitis rats [Elektronische Daten] [Yan Huang, Xiao-Ming Meng, Guo-Lin Jiang, Ya-Ru Yang, Juan Liu, Xiong-Wen Lv, Jun Li] Lipopolysaccharide (LPS), a potent stimulator of inflammatory responses in alveolar macrophages (AMs), activates several intracellular signaling pathways, including mitogen-activated protein kinases (MAPK). In the present study, we investigated the MAPK pathway in AMs of chronic bronchitis (CB) rats. CB was induced by endotracheal instillation of LPS followed by Bacillus Calmette Guerin injection through the caudal vein 1week later. Specific inhibitors were used and protein phosphorylations were detected by Western blot. We found that Genistein (PTK inhibitor) could inhibit protein kinase C (PKC), phosphatidylinositol-3 kinase (PI3K)/protein kinase B (Akt or PKB) MAPK signaling pathway with different degrees, LY294002 (PI3K inhibitor) could not only inhibit phospho-PI3K/Akt expression, but also inhibit p38 and c-Jun NH2-terminal kinases (JNK) phosphorylation. Calphostin C (PKC inhibitor) could inhibit phospho-PKC expression and exerted significant effects on extracellular signal-regulated kinases (ERK) phosphorylation, however, it had no impact on p38 and JNK phosphorylation. These results demonstrated that the LPS mediated signaling pathway of MAPK in AMs of CB rats could be described as follows: PTK-PI3K-Akt-JNK/p38 or PTK-PI3K-PKC-ERK, and PI3K may have a negative regulation on the activation of downstream proteins. Springer Science+Business Media New York, 2014 Alveolar macrophages nationallicence Chronic bronchitis nationallicence Mitogen-activated protein kinase signaling pathway nationallicence AMs : Alveolar macrophages nationallicence CB : Chronic bronchitis nationallicence LPS : Lipopolysaccharide nationallicence BCG : Bacillus Calmette Guerin nationallicence MAPK : Mitogen-activated protein kinases nationallicence PKC : Protein kinase C nationallicence PKA : Protein kinase A nationallicence ERK : Extracellular signal-regulated kinases nationallicence JNK : c-Jun NH2-terminal kinases nationallicence FBS : Fetal bovine serum nationallicence CMC : Carboxymethylcellulose nationallicence BALF : Bronchoalveolar lavage fluid nationallicence PI3K : Phosphatidylinositol-3 kinase nationallicence PTK : Receptor tyrosine kinase nationallicence Huang Yan School of Pharmacy, Anhui Medical University, Tunxi West Road, 230032, Hefei, Anhui, China aut Meng Xiao-Ming School of Pharmacy, Anhui Medical University, Tunxi West Road, 230032, Hefei, Anhui, China aut Jiang Guo-Lin School of Pharmacy, Anhui Medical University, Tunxi West Road, 230032, Hefei, Anhui, China aut Yang Ya-Ru School of Pharmacy, Anhui Medical University, Tunxi West Road, 230032, Hefei, Anhui, China aut Liu Juan School of Pharmacy, Anhui Medical University, Tunxi West Road, 230032, Hefei, Anhui, China aut Lv Xiong-Wen School of Pharmacy, Anhui Medical University, Tunxi West Road, 230032, Hefei, Anhui, China aut Li Jun School of Pharmacy, Anhui Medical University, Tunxi West Road, 230032, Hefei, Anhui, China aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 400/1-2(2015-02-01), 97-105 0300-8177 400:1-2<97 2015 400 11010 https://doi.org/10.1007/s11010-014-2266-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-014-2266-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Huang Yan School of Pharmacy, Anhui Medical University, Tunxi West Road, 230032, Hefei, Anhui, China aut NATIONALLICENCE 700 1- Meng Xiao-Ming School of Pharmacy, Anhui Medical University, Tunxi West Road, 230032, Hefei, Anhui, China aut NATIONALLICENCE 700 1- Jiang Guo-Lin School of Pharmacy, Anhui Medical University, Tunxi West Road, 230032, Hefei, Anhui, China aut NATIONALLICENCE 700 1- Yang Ya-Ru School of Pharmacy, Anhui Medical University, Tunxi West Road, 230032, Hefei, Anhui, China aut NATIONALLICENCE 700 1- Liu Juan School of Pharmacy, Anhui Medical University, Tunxi West Road, 230032, Hefei, Anhui, China aut NATIONALLICENCE 700 1- Lv Xiong-Wen School of Pharmacy, Anhui Medical University, Tunxi West Road, 230032, Hefei, Anhui, China aut NATIONALLICENCE 700 1- Li Jun School of Pharmacy, Anhui Medical University, Tunxi West Road, 230032, Hefei, Anhui, China aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 400/1-2(2015-02-01), 97-105 0300-8177 400:1-2<97 2015 400 11010