caa a22 4500 606236473 CHVBK 20210128101248.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s11010-014-2281-2 doi (NATIONALLICENCE)springer-10.1007/s11010-014-2281-2 MiR-135b promotes proliferation and invasion of osteosarcoma cells via targeting FOXO1 [Elektronische Daten] [Hong Pei, Zhiliang Jin, Shunguang Chen, Xianglun Sun, Jie Yu, Weichun Guo] Recent data strongly suggest the important role of miRNAs in various cancer-related processes. Osteosarcoma (OS) is the most common primary cancer of the bone and usually leads to deaths due to its rapid proliferation and metastasis. Here, we demonstrated that compared with noncancerous bone tissues, miR-135b expression is frequently upregulated in OS specimens, inversely correlated with potential target-FOXO1 expression pattern. Bioinformatics analysis combined with experimental confirmation revealed FOXO1 is a direct target of miR-135b in OS. Functionally, miR-135b inhibitor significantly inhibited OS cells proliferation and invasion. Forced expression of FOXO1 showed the opposite effect, and FOXO1 knockdown abolished the effect of miR-135b inhibitor. Taken together, our data provide compelling evidence that miR-135b functions as an onco-miRNA in OS to promote OS cells proliferation and invasion, and its oncogenic effects are mediated chiefly through targeting FOXO1. Springer Science+Business Media New York, 2014 Osteosarcoma nationallicence miR-135b nationallicence FOXO1 nationallicence Proliferation nationallicence Invasion nationallicence Pei Hong Department of Orthopedics, Renmin Hospital of Wuhan University, Zhangzhidong Road 99#, 430060, Wuhan, Hubei, People's Republic of China aut Jin Zhiliang Department of Orthopedics, Renmin Hospital of Wuhan University, Zhangzhidong Road 99#, 430060, Wuhan, Hubei, People's Republic of China aut Chen Shunguang Department of Orthopedics, Renmin Hospital of Wuhan University, Zhangzhidong Road 99#, 430060, Wuhan, Hubei, People's Republic of China aut Sun Xianglun Department of Orthopedics, Renmin Hospital of Wuhan University, Zhangzhidong Road 99#, 430060, Wuhan, Hubei, People's Republic of China aut Yu Jie Department of Orthopedics, Renmin Hospital of Wuhan University, Zhangzhidong Road 99#, 430060, Wuhan, Hubei, People's Republic of China aut Guo Weichun Department of Orthopedics, Renmin Hospital of Wuhan University, Zhangzhidong Road 99#, 430060, Wuhan, Hubei, People's Republic of China aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 400/1-2(2015-02-01), 245-252 0300-8177 400:1-2<245 2015 400 11010 https://doi.org/10.1007/s11010-014-2281-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-014-2281-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pei Hong Department of Orthopedics, Renmin Hospital of Wuhan University, Zhangzhidong Road 99#, 430060, Wuhan, Hubei, People's Republic of China aut NATIONALLICENCE 700 1- Jin Zhiliang Department of Orthopedics, Renmin Hospital of Wuhan University, Zhangzhidong Road 99#, 430060, Wuhan, Hubei, People's Republic of China aut NATIONALLICENCE 700 1- Chen Shunguang Department of Orthopedics, Renmin Hospital of Wuhan University, Zhangzhidong Road 99#, 430060, Wuhan, Hubei, People's Republic of China aut NATIONALLICENCE 700 1- Sun Xianglun Department of Orthopedics, Renmin Hospital of Wuhan University, Zhangzhidong Road 99#, 430060, Wuhan, Hubei, People's Republic of China aut NATIONALLICENCE 700 1- Yu Jie Department of Orthopedics, Renmin Hospital of Wuhan University, Zhangzhidong Road 99#, 430060, Wuhan, Hubei, People's Republic of China aut NATIONALLICENCE 700 1- Guo Weichun Department of Orthopedics, Renmin Hospital of Wuhan University, Zhangzhidong Road 99#, 430060, Wuhan, Hubei, People's Republic of China aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 400/1-2(2015-02-01), 245-252 0300-8177 400:1-2<245 2015 400 11010