caa a22 4500 606236511 CHVBK 20210128101248.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s11010-014-2270-5 doi (NATIONALLICENCE)springer-10.1007/s11010-014-2270-5 Role of GPR120 in cell motile activity induced by 12- O- tetradecanoylphorbol-13-acetate in liver epithelial WB-F344 cells [Elektronische Daten] [Shuhei Ishii, Miku Hirane, Yuka Kitamura, Shiori Mori, Nobuyuki Fukushima, Kanya Honoki, Toshifumi Tsujiuchi] G-protein-coupled receptor 120 (GPR120) is identified as a G-protein-coupled receptor for unsaturated long-chain free fatty acids that mediates insulin signaling and anti-inflammatory effects. Recently, it has been reported that GPR120 promotes the cell motile activity and angiogenesis in cancer cells. In this study, we assessed the role of GPR120 in the cell motile activity induced by 12-O-tetradecanoylphorbol-13-acetate (TPA) in rat liver epithelial WB-F344 cells. Cells were treated with TPA at a concentration of 5nM for 72h. The expression level of the Gpr120 gene was measured by quantitative real-time RT-PCR analysis. Cells treated with TPA showed the elevated Gpr120 expression, in comparison with untreated cells. In cell motility assays, the cell motile activity of cells treated with TPA was significantly higher than that of untreated cells. To confirm whether GPR120 is involved in the cell motile activity mediated by TPA, we generated GPR120 knockdown cells from WB-F344 cells. The cell motile activity induced by TPA was significantly suppressed by GPR120 knockdown. These results suggest that GPR120 plays an important role in the cell motile activity induced by TPA in WB-F344 cells. Springer Science+Business Media New York, 2014 GPR120 nationallicence 12- O- tetradecanoylphorbol-13-acetate nationallicence Cell motility nationallicence Liver nationallicence Ishii Shuhei Division of Cancer Biology and Bioinformatics, Department of Life Science, Faculty of Science and Engineering, Kinki University, 3-4-1, Kowakae, 577-8502, Higashiosaka, Osaka, Japan aut Hirane Miku Division of Cancer Biology and Bioinformatics, Department of Life Science, Faculty of Science and Engineering, Kinki University, 3-4-1, Kowakae, 577-8502, Higashiosaka, Osaka, Japan aut Kitamura Yuka Division of Cancer Biology and Bioinformatics, Department of Life Science, Faculty of Science and Engineering, Kinki University, 3-4-1, Kowakae, 577-8502, Higashiosaka, Osaka, Japan aut Mori Shiori Division of Cancer Biology and Bioinformatics, Department of Life Science, Faculty of Science and Engineering, Kinki University, 3-4-1, Kowakae, 577-8502, Higashiosaka, Osaka, Japan aut Fukushima Nobuyuki Division of Molecular Neurobiology, Department of Life Science, Faculty of Science and Engineering, Kinki University, 3-4-1, Kowakae, 577-8502, Higashiosaka, Osaka, Japan aut Honoki Kanya Department of Orthopedic Surgery, Nara Medical University, 840 Shijo-cho, 634-8521, Kashihara, Nara, Japan aut Tsujiuchi Toshifumi Division of Cancer Biology and Bioinformatics, Department of Life Science, Faculty of Science and Engineering, Kinki University, 3-4-1, Kowakae, 577-8502, Higashiosaka, Osaka, Japan aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 400/1-2(2015-02-01), 145-151 0300-8177 400:1-2<145 2015 400 11010 https://doi.org/10.1007/s11010-014-2270-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-014-2270-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ishii Shuhei Division of Cancer Biology and Bioinformatics, Department of Life Science, Faculty of Science and Engineering, Kinki University, 3-4-1, Kowakae, 577-8502, Higashiosaka, Osaka, Japan aut NATIONALLICENCE 700 1- Hirane Miku Division of Cancer Biology and Bioinformatics, Department of Life Science, Faculty of Science and Engineering, Kinki University, 3-4-1, Kowakae, 577-8502, Higashiosaka, Osaka, Japan aut NATIONALLICENCE 700 1- Kitamura Yuka Division of Cancer Biology and Bioinformatics, Department of Life Science, Faculty of Science and Engineering, Kinki University, 3-4-1, Kowakae, 577-8502, Higashiosaka, Osaka, Japan aut NATIONALLICENCE 700 1- Mori Shiori Division of Cancer Biology and Bioinformatics, Department of Life Science, Faculty of Science and Engineering, Kinki University, 3-4-1, Kowakae, 577-8502, Higashiosaka, Osaka, Japan aut NATIONALLICENCE 700 1- Fukushima Nobuyuki Division of Molecular Neurobiology, Department of Life Science, Faculty of Science and Engineering, Kinki University, 3-4-1, Kowakae, 577-8502, Higashiosaka, Osaka, Japan aut NATIONALLICENCE 700 1- Honoki Kanya Department of Orthopedic Surgery, Nara Medical University, 840 Shijo-cho, 634-8521, Kashihara, Nara, Japan aut NATIONALLICENCE 700 1- Tsujiuchi Toshifumi Division of Cancer Biology and Bioinformatics, Department of Life Science, Faculty of Science and Engineering, Kinki University, 3-4-1, Kowakae, 577-8502, Higashiosaka, Osaka, Japan aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 400/1-2(2015-02-01), 145-151 0300-8177 400:1-2<145 2015 400 11010