caa a22 4500 606236554 CHVBK 20210128101248.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s11010-014-2265-2 doi (NATIONALLICENCE)springer-10.1007/s11010-014-2265-2 VEGF-mediated suppression of cell proliferation and invasion by miR-410 in osteosarcoma [Elektronische Daten] [Dong Zhao, Peng Jia, Wenliang Wang, Guangling Zhang] MicroRNAs (miRNAs) are small non-coding RNAs that post-transcriptionally regulate gene expression. The aberrant expression of miRNA has become a major focus in cancer research. This study aimed to investigate the importance of miR-410 in the diagnosis and therapy of osteosarcoma (OS). Western blot analysis showed that the expression of VEGF was higher in Saos-2 and MG-63 cells than that in three other OS cell lines. We also found that miR-410 was lowly expressed and inversely correlated with VEGF expression in OS specimens. Over-expression of miR-410 had a greater repression on VEGF expression than othercandidate VEGF-targeting miRNAs. Luciferase reporter assay demonstrated that miR-410 directly decreased VEGF expression by targeting its 3′-untranslated region. Further investigation demonstrated the regulation of miR-410 in OS cells via VEGF. In vitro MTT assay, Transwell, and flow cytometry showed that transfection of the miR-410 expression plasmid inhibited cell proliferation and contributed to apoptosis in OS cells. Moreover, restoration of VEGF reversed the effect of miR-410 on OS cells, and upregulated the expression of phosphorylated AKT. Finally, overexpression of miR-410 also showed a negative effect on tumor growth in vivo. Our findings suggest a cooperative relationship between miR-410 and VEGF in OS cell regulation. This information may help researchers to better understand miRNA regulation in cancer and provide a rationale for developing miRNA-based strategies for OS treatment. Springer Science+Business Media New York, 2014 Osteosarcoma nationallicence miR-410 nationallicence VEGF nationallicence Proliferation nationallicence Invasion nationallicence Zhao Dong Department of Orthopedics, Affiliated Hospital, Logistics University of Chinese People's Armed Police Forces, No. 220 Chenglin Road, Hedong district, 300162, Tianjin, China aut Jia Peng Department of Orthopedics, Affiliated Hospital, Logistics University of Chinese People's Armed Police Forces, No. 220 Chenglin Road, Hedong district, 300162, Tianjin, China aut Wang Wenliang Department of Orthopedics, Affiliated Hospital, Logistics University of Chinese People's Armed Police Forces, No. 220 Chenglin Road, Hedong district, 300162, Tianjin, China aut Zhang Guangling School of Basic Medical Sciences, Hebei United University, No. 57 Jianshe Road, 063000, Tangshan City, Hebei Province, China aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 400/1-2(2015-02-01), 87-95 0300-8177 400:1-2<87 2015 400 11010 https://doi.org/10.1007/s11010-014-2265-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-014-2265-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhao Dong Department of Orthopedics, Affiliated Hospital, Logistics University of Chinese People's Armed Police Forces, No. 220 Chenglin Road, Hedong district, 300162, Tianjin, China aut NATIONALLICENCE 700 1- Jia Peng Department of Orthopedics, Affiliated Hospital, Logistics University of Chinese People's Armed Police Forces, No. 220 Chenglin Road, Hedong district, 300162, Tianjin, China aut NATIONALLICENCE 700 1- Wang Wenliang Department of Orthopedics, Affiliated Hospital, Logistics University of Chinese People's Armed Police Forces, No. 220 Chenglin Road, Hedong district, 300162, Tianjin, China aut NATIONALLICENCE 700 1- Zhang Guangling School of Basic Medical Sciences, Hebei United University, No. 57 Jianshe Road, 063000, Tangshan City, Hebei Province, China aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 400/1-2(2015-02-01), 87-95 0300-8177 400:1-2<87 2015 400 11010