caa a22 4500 606236562 CHVBK 20210128101248.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s11010-014-2283-0 doi (NATIONALLICENCE)springer-10.1007/s11010-014-2283-0 Glutathione depletion and acute exercise increase O -GlcNAc protein modification in rat skeletal muscle [Elektronische Daten] [Tina Peternelj, Susan Marsh, Natalie Strobel, Aya Matsumoto, David Briskey, Vincent Dalbo, Patrick Tucker, Jeff Coombes] Post-translational modification of intracellular proteins with O-linked β-N-acetylglucosamine (O-GlcNAc) profoundly affects protein structure, function, and metabolism. Although many skeletal muscle proteins are O-GlcNAcylated, the modification has not been extensively studied in this tissue, especially in the context of exercise. This study investigated the effects of glutathione depletion and acute exercise on O-GlcNAc protein modification in rat skeletal muscle. Diethyl maleate (DEM) was used to deplete intracellular glutathione and rats were subjected to a treadmill run. White gastrocnemius and soleus muscles were analyzed for glutathione status, O-GlcNAc and O-GlcNAc transferase (OGT) protein levels, and mRNA expression of OGT, O-GlcNAcase and glutamine:fructose-6-phosphate amidotransferase. DEM and exercise both reduced intracellular glutathione and increased O-GlcNAc. DEM upregulated OGT protein expression. The effects of the interventions were significant 4h after exercise (P<0.05). The changes in the mRNA levels of O-GlcNAc enzymes were different in the two muscles, potentially resulting from different rates of oxidative stress and metabolic demands between the muscle types. These findings indicate that oxidative environment promotes O-GlcNAcylation in skeletal muscle and suggest an interrelationship between cellular redox state and O-GlcNAc protein modification. This could represent one mechanism underlying cellular adaptation to oxidative stress and health benefits of exercise. Springer Science+Business Media New York, 2014 Diethyl maleate nationallicence Treadmill run nationallicence White gastrocnemius nationallicence Soleus nationallicence O -GlcNAc nationallicence Peternelj Tina Antioxidant Research Group, School of Human Movement Studies, The University of Queensland, St Lucia, 4072, Brisbane, QLD, Australia aut Marsh Susan Section of Clinical Pharmacology, College of Pharmacy, Washington State University, Spokane, WA, USA aut Strobel Natalie Antioxidant Research Group, School of Human Movement Studies, The University of Queensland, St Lucia, 4072, Brisbane, QLD, Australia aut Matsumoto Aya Baker IDI Heart and Diabetes Institute, Melbourne, VIC, Australia aut Briskey David Antioxidant Research Group, School of Human Movement Studies, The University of Queensland, St Lucia, 4072, Brisbane, QLD, Australia aut Dalbo Vincent Clinical Biochemistry Laboratory, Central Queensland University, Rockhampton, QLD, Australia aut Tucker Patrick Clinical Biochemistry Laboratory, Central Queensland University, Rockhampton, QLD, Australia aut Coombes Jeff Antioxidant Research Group, School of Human Movement Studies, The University of Queensland, St Lucia, 4072, Brisbane, QLD, Australia aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 400/1-2(2015-02-01), 265-275 0300-8177 400:1-2<265 2015 400 11010 https://doi.org/10.1007/s11010-014-2283-0 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-014-2283-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Peternelj Tina Antioxidant Research Group, School of Human Movement Studies, The University of Queensland, St Lucia, 4072, Brisbane, QLD, Australia aut NATIONALLICENCE 700 1- Marsh Susan Section of Clinical Pharmacology, College of Pharmacy, Washington State University, Spokane, WA, USA aut NATIONALLICENCE 700 1- Strobel Natalie Antioxidant Research Group, School of Human Movement Studies, The University of Queensland, St Lucia, 4072, Brisbane, QLD, Australia aut NATIONALLICENCE 700 1- Matsumoto Aya Baker IDI Heart and Diabetes Institute, Melbourne, VIC, Australia aut NATIONALLICENCE 700 1- Briskey David Antioxidant Research Group, School of Human Movement Studies, The University of Queensland, St Lucia, 4072, Brisbane, QLD, Australia aut NATIONALLICENCE 700 1- Dalbo Vincent Clinical Biochemistry Laboratory, Central Queensland University, Rockhampton, QLD, Australia aut NATIONALLICENCE 700 1- Tucker Patrick Clinical Biochemistry Laboratory, Central Queensland University, Rockhampton, QLD, Australia aut NATIONALLICENCE 700 1- Coombes Jeff Antioxidant Research Group, School of Human Movement Studies, The University of Queensland, St Lucia, 4072, Brisbane, QLD, Australia aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 400/1-2(2015-02-01), 265-275 0300-8177 400:1-2<265 2015 400 11010