caa a22 4500 606236597 CHVBK 20210128101248.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s11010-014-2282-1 doi (NATIONALLICENCE)springer-10.1007/s11010-014-2282-1 Lipidomic analysis of molecular cardiolipin species in livers exposed to ischemia/reperfusion [Elektronische Daten] [Jan-Christian Martens, Gerburg Keilhoff, Walter Halangk, Thomas Wartmann, Andreas Gardemann, Ilona Päge, Lorenz Schild] Transient hepatic ischemia can cause significant liver injury. A central and early event in ischemia/reperfusion (I/R) injury is the impairment of mitochondria. The phospholipid cardiolipin (CL) is required for efficient mitochondrial function. The aim of this study was to analyze composition, content, and oxidation of CL in dependence of I/R stress. Therefore, we exposed rat livers to 20min ischemia by interrupting the perfusion with Krebs-Ringer solution in situ. Tissue histology as well as increased activities of LDH, GLDH, and ASAT analysed in the efflux after 50min reperfusion indicated impairment of the liver. For the analysis of local CL distribution the liver homogenate was separated according to density into 11 fractions. The fractions displayed different contents of CL and citrate synthase peaking at density of about 1.07g/cm3. Among the fractions, the distribution of molecular CL species significantly differed. I/R caused loss of about 30% CL and 17% citrate synthase activity. Further, I/R shifted the CL and citrate synthase activity profile toward lower densities. Oxidized CL was exclusively found in fractions with high CL and citrate synthase content after I/R stress. I/R treatment caused significant changes in the distribution of molecular CL species. Our data demonstrate that I/R causes significant decrease in CL content and increase of oxidized CL that may be of impact for impairment of mitochondrial function by I/R. These results lead to the suggestion that strategies supporting anti-oxidative defence and CL synthesis may be beneficial to reduce I/R injury of the liver. Springer Science+Business Media New York, 2014 Liver nationallicence Ischemia/reperfusion nationallicence Oxidative stress nationallicence Cardiolipin nationallicence Martens Jan-Christian Department of Pathological Biochemistry, Medical Faculty, Institute of Clinical Chemistry and Pathological Biochemistry, Otto-von-Guericke-University, Magdeburg, Leipziger Str. 44, Magdeburg, Germany aut Keilhoff Gerburg Institute of Biochemistry and Cell Biology, Otto-von-Guericke-University, Magdeburg, Magdeburg, Germany aut Halangk Walter Division of Experimental Surgery, Department of Surgery, Otto-von-Guericke-University, Magdeburg, Magdeburg, Germany aut Wartmann Thomas Division of Experimental Surgery, Department of Surgery, Otto-von-Guericke-University, Magdeburg, Magdeburg, Germany aut Gardemann Andreas Department of Pathological Biochemistry, Medical Faculty, Institute of Clinical Chemistry and Pathological Biochemistry, Otto-von-Guericke-University, Magdeburg, Leipziger Str. 44, Magdeburg, Germany aut Päge Ilona Department of Pathological Biochemistry, Medical Faculty, Institute of Clinical Chemistry and Pathological Biochemistry, Otto-von-Guericke-University, Magdeburg, Leipziger Str. 44, Magdeburg, Germany aut Schild Lorenz Department of Pathological Biochemistry, Medical Faculty, Institute of Clinical Chemistry and Pathological Biochemistry, Otto-von-Guericke-University, Magdeburg, Leipziger Str. 44, Magdeburg, Germany aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 400/1-2(2015-02-01), 253-263 0300-8177 400:1-2<253 2015 400 11010 https://doi.org/10.1007/s11010-014-2282-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-014-2282-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Martens Jan-Christian Department of Pathological Biochemistry, Medical Faculty, Institute of Clinical Chemistry and Pathological Biochemistry, Otto-von-Guericke-University, Magdeburg, Leipziger Str. 44, Magdeburg, Germany aut NATIONALLICENCE 700 1- Keilhoff Gerburg Institute of Biochemistry and Cell Biology, Otto-von-Guericke-University, Magdeburg, Magdeburg, Germany aut NATIONALLICENCE 700 1- Halangk Walter Division of Experimental Surgery, Department of Surgery, Otto-von-Guericke-University, Magdeburg, Magdeburg, Germany aut NATIONALLICENCE 700 1- Wartmann Thomas Division of Experimental Surgery, Department of Surgery, Otto-von-Guericke-University, Magdeburg, Magdeburg, Germany aut NATIONALLICENCE 700 1- Gardemann Andreas Department of Pathological Biochemistry, Medical Faculty, Institute of Clinical Chemistry and Pathological Biochemistry, Otto-von-Guericke-University, Magdeburg, Leipziger Str. 44, Magdeburg, Germany aut NATIONALLICENCE 700 1- Päge Ilona Department of Pathological Biochemistry, Medical Faculty, Institute of Clinical Chemistry and Pathological Biochemistry, Otto-von-Guericke-University, Magdeburg, Leipziger Str. 44, Magdeburg, Germany aut NATIONALLICENCE 700 1- Schild Lorenz Department of Pathological Biochemistry, Medical Faculty, Institute of Clinical Chemistry and Pathological Biochemistry, Otto-von-Guericke-University, Magdeburg, Leipziger Str. 44, Magdeburg, Germany aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 400/1-2(2015-02-01), 253-263 0300-8177 400:1-2<253 2015 400 11010