caa a22 4500 606236651 CHVBK 20210128101249.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s11010-014-2228-7 doi (NATIONALLICENCE)springer-10.1007/s11010-014-2228-7 Oleanolic acid suppresses the proliferation of lung carcinoma cells by miR-122/Cyclin G1/MEF2D axis [Elektronische Daten] [Xiaoming Zhao, Ming Liu, Daotang Li] Oleanolic acid (OA) is a natural compound from plants with anti-tumor activities. However, the mechanism of the inhibitory effect of OA on cell cycle progression has not been completely explored. We employed several lung carcinoma cell lines to investigate the cell cycle-related molecular pathway affected by OA. The data revealed that OA suppressed the proliferation of lung cancer cells in both dose- and time-dependent manners, along with an increase in miR-122 abundance. The suppression of miR-122 abolished the effect of OA on lung cancer cells. CCNG1 and MEF2D, two putative miR-122 targets, were found to be downregulated by OA treatment. Restoring their expression counteracted the effect of OA on lung carcinoma cells. OA was further shown to induce the expression of miR-122-regulating transcriptional factors in lung cancer cells. Collectively, OA induced cell cycle arrest in lung cancer cells through miR-122/Cyclin G1/MEF2D pathway. This finding may contribute to the understanding of the molecular mechanism of OA's anti-tumor activity. Springer Science+Business Media New York, 2014 CCNG1 nationallicence miR-122 nationallicence MEF2D nationallicence Lung cancer nationallicence Oleanolic acid nationallicence Zhao Xiaoming Department of Thoracic Surgery, Shandong Cancer Hospital and Institute, 440 Jiyan Road, 250117, Jinan, Shandong, People's Republic of China aut Liu Ming Department of Thoracic Surgery, Shandong Cancer Hospital and Institute, 440 Jiyan Road, 250117, Jinan, Shandong, People's Republic of China aut Li Daotang Department of Thoracic Surgery, Shandong Cancer Hospital and Institute, 440 Jiyan Road, 250117, Jinan, Shandong, People's Republic of China aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 400/1-2(2015-02-01), 1-7 0300-8177 400:1-2<1 2015 400 11010 https://doi.org/10.1007/s11010-014-2228-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-014-2228-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhao Xiaoming Department of Thoracic Surgery, Shandong Cancer Hospital and Institute, 440 Jiyan Road, 250117, Jinan, Shandong, People's Republic of China aut NATIONALLICENCE 700 1- Liu Ming Department of Thoracic Surgery, Shandong Cancer Hospital and Institute, 440 Jiyan Road, 250117, Jinan, Shandong, People's Republic of China aut NATIONALLICENCE 700 1- Li Daotang Department of Thoracic Surgery, Shandong Cancer Hospital and Institute, 440 Jiyan Road, 250117, Jinan, Shandong, People's Republic of China aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 400/1-2(2015-02-01), 1-7 0300-8177 400:1-2<1 2015 400 11010