caa a22 4500 60623666X CHVBK 20210128101249.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s11010-015-2349-7 doi (NATIONALLICENCE)springer-10.1007/s11010-015-2349-7 The BM2 protein of influenza B virus interacts with p53 and inhibits its transcriptional and apoptotic activities [Elektronische Daten] [H. Zhang, H. Yu, J. Wang, M. Zhang, X. Wang, W. Ahmad, M. Duan, Z. Guan] The influenza virus integral membrane proteins BM2 (M2 of influenza B virus) and A/M2 (M2 of influenza A virus) functions as an ion channel, important for virus uncoating in endosomes of virus-infected cells and essential for viral replication. M2 ion channel activity is also required to stimulate NLRP3 inflammasome activation by perturbing ionic concentrations in the Golgi. In the present study, we have investigated further the interaction between BM2 and p53 to confirm our previous studies using yeast two-hybrid assays. The interaction between BM2 and p53 was confirmed by GST pull-down, co-immunoprecipitation assays, and confocal microscopy. Expression of BM2 results in down-regulation of p53 mRNA and protein expression in a dose-dependent manner. In addition, we demonstrated that exogenous expression of BM2 functionally blocked p53-mediated transcriptional activity and apoptosis by luciferase reporter assay and TUNEL assay, respectively. Together, the present results indicate that BM2 is able to functionally interact with p53, and provide valuable insights into the modulation of p53 functions by influenza virus. Springer Science+Business Media New York, 2015 BM2 nationallicence p53 nationallicence Protein-protein interaction nationallicence Apoptosis nationallicence Zhang H. Key Lab of Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, 130062, Changchun, People's Republic of China aut Yu H. Key Lab of Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, 130062, Changchun, People's Republic of China aut Wang J. Key Lab of Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, 130062, Changchun, People's Republic of China aut Zhang M. Key Lab of Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, 130062, Changchun, People's Republic of China aut Wang X. Key Lab of Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, 130062, Changchun, People's Republic of China aut Ahmad W. Key Lab of Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, 130062, Changchun, People's Republic of China aut Duan M. Key Lab of Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, 130062, Changchun, People's Republic of China aut Guan Z. Key Lab of Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, 130062, Changchun, People's Republic of China aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 403/1-2(2015-05-01), 187-197 0300-8177 403:1-2<187 2015 403 11010 https://doi.org/10.1007/s11010-015-2349-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2349-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang H. Key Lab of Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, 130062, Changchun, People's Republic of China aut NATIONALLICENCE 700 1- Yu H. Key Lab of Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, 130062, Changchun, People's Republic of China aut NATIONALLICENCE 700 1- Wang J. Key Lab of Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, 130062, Changchun, People's Republic of China aut NATIONALLICENCE 700 1- Zhang M. Key Lab of Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, 130062, Changchun, People's Republic of China aut NATIONALLICENCE 700 1- Wang X. Key Lab of Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, 130062, Changchun, People's Republic of China aut NATIONALLICENCE 700 1- Ahmad W. Key Lab of Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, 130062, Changchun, People's Republic of China aut NATIONALLICENCE 700 1- Duan M. Key Lab of Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, 130062, Changchun, People's Republic of China aut NATIONALLICENCE 700 1- Guan Z. Key Lab of Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, 130062, Changchun, People's Republic of China aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 403/1-2(2015-05-01), 187-197 0300-8177 403:1-2<187 2015 403 11010