caa a22 4500 606236694 CHVBK 20210128101249.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s11010-015-2340-3 doi (NATIONALLICENCE)springer-10.1007/s11010-015-2340-3 Apigenin inhibits PMA-induced expression of pro-inflammatory cytokines andAP-1 factors in A549 cells [Elektronische Daten] [Rajeshwari Patil, R. Babu, M. Naveen Kumar, K. Kiran Kumar, Shubha Hegde, Govindarajan Ramesh, S. Chidananda Sharma] Acute and chronic alveolar or bronchial inflammation is thought to be central to the pathogenesis of many respiratory disorders. Cytokines and granulocyte macrophage colony-stimulating factors (GM-CSF) play an important role in chronic inflammation. Activator protein-1 (AP-1) the superfamily of transcription factors is involved in proliferation, differentiation, apoptosis, and transformation including inflammation. Understanding the function and regulation of proinflammatory factors involved in inflammation may provide the novel therapeutic strategies in the treatment of inflammatory diseases. Our aim of the present study is to investigate the pro-inflammatory cytokines and pattern of AP-1 factors expressed during activation of lung adenocarcinoma A549 cells by Phorbol-12-myristate-13-acetate (PMA) and to understand the anti-inflammatory effect of apigenin. A549 cells were treated with and without PMA or apigenin, and the cell viability was assessed by MTT assay. Expressions of inflammatory mediators and different AP-1 factors were analyzed by semi-quantitative RT-PCR. IL-6 protein secreted was analyzed by ELISA, and expressions of IL-1β, c-Jun, and c-Fos proteins were analyzed by Western blotting. Activation of A549 cells by PMA, induced the expression of pro-inflammatory cytokine (IL-1β, IL-2, IL-6, IL-8, and TNF-α) mRNAs and secretion of IL-6 and the expression of specific AP-1 factors (c-Jun, c-Fos, and Fra-1). Treatment of cells with apigenin, significantly inhibited PMA-stimulated mRNA expression of above pro-inflammatory cytokines, AP-1 factors, cyclooxygenase-2, and secretion of IL-6 protein. Results suggested that the AP-1 factors may be involved in inflammation and apigenin has anti-inflammatory effect, which may be useful for therapeutic management of lung inflammatory diseases. Springer Science+Business Media New York, 2015 Phorbol-12-myristate-13-acetate nationallicence Apigenin nationallicence Cytokines nationallicence AP-1 nationallicence A549 nationallicence qRT-PCR nationallicence Patil Rajeshwari Department of Microbiology and Biotechnology, Bangalore University, 560 056, Bangalore, Karnataka, India aut Babu R. Department of Microbiology and Biotechnology, Bangalore University, 560 056, Bangalore, Karnataka, India aut Naveen Kumar M. Department of Microbiology and Biotechnology, Bangalore University, 560 056, Bangalore, Karnataka, India aut Kiran Kumar K. Department of Microbiology and Biotechnology, Bangalore University, 560 056, Bangalore, Karnataka, India aut Hegde Shubha Department of Microbiology and Biotechnology, Bangalore University, 560 056, Bangalore, Karnataka, India aut Ramesh Govindarajan Department of Biology and Center for Biotechnology and Biomedical Sciences, Norfolk State University, Norfolk, VA, USA aut Chidananda Sharma S. Department of Microbiology and Biotechnology, Bangalore University, 560 056, Bangalore, Karnataka, India aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 403/1-2(2015-05-01), 95-106 0300-8177 403:1-2<95 2015 403 11010 https://doi.org/10.1007/s11010-015-2340-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2340-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Patil Rajeshwari Department of Microbiology and Biotechnology, Bangalore University, 560 056, Bangalore, Karnataka, India aut NATIONALLICENCE 700 1- Babu R. Department of Microbiology and Biotechnology, Bangalore University, 560 056, Bangalore, Karnataka, India aut NATIONALLICENCE 700 1- Naveen Kumar M. Department of Microbiology and Biotechnology, Bangalore University, 560 056, Bangalore, Karnataka, India aut NATIONALLICENCE 700 1- Kiran Kumar K. Department of Microbiology and Biotechnology, Bangalore University, 560 056, Bangalore, Karnataka, India aut NATIONALLICENCE 700 1- Hegde Shubha Department of Microbiology and Biotechnology, Bangalore University, 560 056, Bangalore, Karnataka, India aut NATIONALLICENCE 700 1- Ramesh Govindarajan Department of Biology and Center for Biotechnology and Biomedical Sciences, Norfolk State University, Norfolk, VA, USA aut NATIONALLICENCE 700 1- Chidananda Sharma S. Department of Microbiology and Biotechnology, Bangalore University, 560 056, Bangalore, Karnataka, India aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 403/1-2(2015-05-01), 95-106 0300-8177 403:1-2<95 2015 403 11010