caa a22 4500 606236759 CHVBK 20210128101249.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s11010-015-2339-9 doi (NATIONALLICENCE)springer-10.1007/s11010-015-2339-9 Glaucine inhibits breast cancer cell migration and invasion by inhibiting MMP-9 gene expression through the suppression of NF-κB activation [Elektronische Daten] [Hyereen Kang, Sung-Wuk Jang, Jhang Pak, Sungbo Shim] Matrix metalloproteinase-9 (MMP-9) plays a central role in the invasion and metastasis of various types of cancer cells. Here, we demonstrate that glaucine, an alkaloid isolated from the plant Corydalis turtschaninovii tuber (Papaveraceae), can inhibit the migration and invasion of human breast cancer cells. We further show that glaucine significantly blocks phorbol 12-myristate 13-acetate (PMA)-induced MMP-9 expression and activity in a dose-dependent manner. Results from reporter gene and electrophoretic mobility shift assays revealed that glaucine inhibits MMP-9 expression by suppressing activation of the nuclear transcription factor nuclear factor-κB (NF-κB). Moreover, glaucine attenuates PMA-induced IκBα degradation and nuclear translocation of NF-κB. Finally, we also found that glaucine inhibits invasion and MMP-9 expression in the highly metastatic MDA-MB-231 breast cancer cell line. Taken together, our findings indicate that the MMP-9 inhibitory activity of glaucine and its abilities to attenuate IκBα and NF-κB activities may be therapeutically useful as a novel means of controlling breast cancer growth and invasiveness. The Author(s), 2015 Invasion nationallicence Migration nationallicence MMPs nationallicence Natural compound nationallicence Breast cancer nationallicence Kang Hyereen Department of Biomedical Sciences, University of Ulsan College of Medicine, 138-736, Seoul, Republic of Korea aut Jang Sung-Wuk Department of Biomedical Sciences, University of Ulsan College of Medicine, 138-736, Seoul, Republic of Korea aut Pak Jhang Asan Institute for Life Sciences, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Republic of Korea aut Shim Sungbo Department of Biomedical Sciences, University of Ulsan College of Medicine, 138-736, Seoul, Republic of Korea aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 403/1-2(2015-05-01), 85-94 0300-8177 403:1-2<85 2015 403 11010 https://doi.org/10.1007/s11010-015-2339-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2339-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kang Hyereen Department of Biomedical Sciences, University of Ulsan College of Medicine, 138-736, Seoul, Republic of Korea aut NATIONALLICENCE 700 1- Jang Sung-Wuk Department of Biomedical Sciences, University of Ulsan College of Medicine, 138-736, Seoul, Republic of Korea aut NATIONALLICENCE 700 1- Pak Jhang Asan Institute for Life Sciences, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Republic of Korea aut NATIONALLICENCE 700 1- Shim Sungbo Department of Biomedical Sciences, University of Ulsan College of Medicine, 138-736, Seoul, Republic of Korea aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 403/1-2(2015-05-01), 85-94 0300-8177 403:1-2<85 2015 403 11010