caa a22 4500 606236791 CHVBK 20210128101249.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s11010-015-2348-8 doi (NATIONALLICENCE)springer-10.1007/s11010-015-2348-8 Multiple protective mechanisms of alpha-lipoic acid in oxidation, apoptosis and inflammation against hydrogen peroxide induced toxicity in human lymphocytes [Elektronische Daten] [Mahban Rahimifard, Mona Navaei-Nigjeh, Maryam Baeeri, Faheem Maqbool, Mohammad Abdollahi] The naturally antioxidant and coenzyme, alpha-lipoic acid (α-LA), has gained considerable attention regarding different functions and therapeutically effective in treating oxidative stress-associated diseases in the human body. This study was designed to examine the protective effect of α-LA against H2O2-induced oxidative stress and apoptosis in human lymphoid cells. Human peripheral blood lymphocytes were preincubated with α-LA and then exposed to H2O2. After that, the viability of the cells, rate of apoptosis, oxidative stress biomarkers such as reactive oxygen species (ROS) and level of lipid peroxidation (LPO), and also tumor necrosis factor-α (TNF-α) were studied. Pretreatment of lymphocytes with α-LA, dramatically enhanced viability of the cells and decreased apoptosis. Investigation of caspases gives a clear picture of the mechanism by which α-LA decreases ROS and causes a reduction in apoptosis through caspase-9-dependent mitochondrial pathway. Furthermore, α-LA dose dependently decreased oxidative stress by a reduction in level of LPO, and the dose of 1000µM indicates a significant decrease (p<0.01) in TNF-α level. Collectively, the present data show that α-LA is an ideal compound which has profound protective effects on oxidation, inflammation, and apoptosis. As a result, α-LA may indicate a new way toward the development of antioxidant therapy. Springer Science+Business Media New York, 2015 Alpha-lipoic acid nationallicence Human lymphoid cells nationallicence H2O2 nationallicence Oxidative stress nationallicence Apoptosis nationallicence Inflammation nationallicence Rahimifard Mahban Toxicology and Poisoning Research Center, Department of Toxicology and Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, 1417614411, Tehran, Iran aut Navaei-Nigjeh Mona Toxicology and Poisoning Research Center, Department of Toxicology and Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, 1417614411, Tehran, Iran aut Baeeri Maryam Toxicology and Poisoning Research Center, Department of Toxicology and Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, 1417614411, Tehran, Iran aut Maqbool Faheem Toxicology and Poisoning Research Center, Department of Toxicology and Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, 1417614411, Tehran, Iran aut Abdollahi Mohammad Toxicology and Poisoning Research Center, Department of Toxicology and Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, 1417614411, Tehran, Iran aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 403/1-2(2015-05-01), 179-186 0300-8177 403:1-2<179 2015 403 11010 https://doi.org/10.1007/s11010-015-2348-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2348-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rahimifard Mahban Toxicology and Poisoning Research Center, Department of Toxicology and Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, 1417614411, Tehran, Iran aut NATIONALLICENCE 700 1- Navaei-Nigjeh Mona Toxicology and Poisoning Research Center, Department of Toxicology and Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, 1417614411, Tehran, Iran aut NATIONALLICENCE 700 1- Baeeri Maryam Toxicology and Poisoning Research Center, Department of Toxicology and Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, 1417614411, Tehran, Iran aut NATIONALLICENCE 700 1- Maqbool Faheem Toxicology and Poisoning Research Center, Department of Toxicology and Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, 1417614411, Tehran, Iran aut NATIONALLICENCE 700 1- Abdollahi Mohammad Toxicology and Poisoning Research Center, Department of Toxicology and Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, 1417614411, Tehran, Iran aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 403/1-2(2015-05-01), 179-186 0300-8177 403:1-2<179 2015 403 11010