caa a22 4500 606236848 CHVBK 20210128101250.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s11010-015-2351-0 doi (NATIONALLICENCE)springer-10.1007/s11010-015-2351-0 Exercise-induced regulation of key factors in substrate choice and gluconeogenesis in mouse liver [Elektronische Daten] [Jakob Knudsen, Rasmus Biensø, Helle Hassing, Anne Jakobsen, Henriette Pilegaard] As the demand for hepatic glucose production increases during exercise, regulation of liver substrate choice and gluconeogenic activity becomes essential. The aim of the present study was to investigate the effect of a single exercise bout on gluconeogenic protein content and regulation of enzymes involved in substrate utilization in the liver. Mice were subjected to 1h of treadmill exercise, and livers were removed immediately, 4 or 10h after exercise. Glucose-6-phosphatase (G6Pase) and phosphoenolpyruvate carboxylase (PEPCK) mRNA contents in theliver increased immediately after exercise, while the PEPCK protein content increased at 10h of recovery. Furthermore, 5′AMP-activated protein kinase (AMPK), acetyl-CoA carboxylase (ACC), and pyruvate dehydrogenase (PDH)-E1α Ser293 phosphorylations decreased immediately after exercise. In addition, PDH kinase 4 (PDK4) mRNA and protein content increased immediately after exercise and at 10h of recovery, respectively. These findings suggest that acute changes in PEPCK and G6Pase protein contents do not contribute to the regulation of gluconeogenic enzyme activity during 1h of non-exhaustive exercise. In addition, the observation that PDH-E1α, AMPK, and ACC phosphorylation decreased immediately after exercise may indicate that carbohydrates rather than fatty acids are utilized for oxidation in the liver during non-exhaustive exercise. Springer Science+Business Media New York, 2015 AMPK nationallicence ACC nationallicence PDK4 nationallicence PDH nationallicence Exercise nationallicence Gluconeogenesis nationallicence Knudsen Jakob Centre of Inflammation and Metabolism, The August Krogh Centre, Department of Biology, University of Copenhagen, Universitetsparken 13 4th floor, 2100, Copenhagen, Denmark aut Biensø Rasmus Centre of Inflammation and Metabolism, The August Krogh Centre, Department of Biology, University of Copenhagen, Universitetsparken 13 4th floor, 2100, Copenhagen, Denmark aut Hassing Helle Centre of Inflammation and Metabolism, The August Krogh Centre, Department of Biology, University of Copenhagen, Universitetsparken 13 4th floor, 2100, Copenhagen, Denmark aut Jakobsen Anne Centre of Inflammation and Metabolism, The August Krogh Centre, Department of Biology, University of Copenhagen, Universitetsparken 13 4th floor, 2100, Copenhagen, Denmark aut Pilegaard Henriette Centre of Inflammation and Metabolism, The August Krogh Centre, Department of Biology, University of Copenhagen, Universitetsparken 13 4th floor, 2100, Copenhagen, Denmark aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 403/1-2(2015-05-01), 209-217 0300-8177 403:1-2<209 2015 403 11010 https://doi.org/10.1007/s11010-015-2351-0 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2351-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Knudsen Jakob Centre of Inflammation and Metabolism, The August Krogh Centre, Department of Biology, University of Copenhagen, Universitetsparken 13 4th floor, 2100, Copenhagen, Denmark aut NATIONALLICENCE 700 1- Biensø Rasmus Centre of Inflammation and Metabolism, The August Krogh Centre, Department of Biology, University of Copenhagen, Universitetsparken 13 4th floor, 2100, Copenhagen, Denmark aut NATIONALLICENCE 700 1- Hassing Helle Centre of Inflammation and Metabolism, The August Krogh Centre, Department of Biology, University of Copenhagen, Universitetsparken 13 4th floor, 2100, Copenhagen, Denmark aut NATIONALLICENCE 700 1- Jakobsen Anne Centre of Inflammation and Metabolism, The August Krogh Centre, Department of Biology, University of Copenhagen, Universitetsparken 13 4th floor, 2100, Copenhagen, Denmark aut NATIONALLICENCE 700 1- Pilegaard Henriette Centre of Inflammation and Metabolism, The August Krogh Centre, Department of Biology, University of Copenhagen, Universitetsparken 13 4th floor, 2100, Copenhagen, Denmark aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 403/1-2(2015-05-01), 209-217 0300-8177 403:1-2<209 2015 403 11010