caa a22 4500 606236864 CHVBK 20210128101250.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s11010-015-2337-y doi (NATIONALLICENCE)springer-10.1007/s11010-015-2337-y Unrevealing the role of P-protein on melanosome biology and structure, using siRNA-mediated down regulation of OCA2 [Elektronische Daten] [Sangjoo Park, V. Morya, Dong Nguyen, Birendra Singh, Hyang-Bok Lee, Eun-Ki Kim] The pink-eyed dilution protein (P-protein) plays a critical role in melanin synthesis in melanocytes and retinal pigment epithelium cells. Mutation in this protein may cause complete or partial albinism. Role of the P-protein ranges in melanin synthesis to maturation and trafficking of the melanosomes. The aim of the present study was to evaluate the effect of P-protein inhibition on melanosome biology by comparing the shape, size, count, and types of melanosomes in melan-a melanocytes. The cells were extensively examined by the transmission electron microscopy. The P-protein inhibition was carried by P-protein-siRNA transfection to melan-a melanocytes, B16F10 mouse melanoma, and melan-p1 cells. Measurement of melanin contents, cellular tyrosinase, and different tyrosinase related proteins were also determined to investigate the effect of P-protein siRNA transfection on melanocytes. Results suggested that the inhibition of P-protein can significantly change the melanosomal morphology, types and their respective numbers, and provided a novel strategy for the control of melanin synthesis. Springer Science+Business Media New York, 2015 Pink-eyed dilution (P) protein nationallicence Melanosome nationallicence siRNA transfection nationallicence Melanin nationallicence Transmission electron microscopy nationallicence Park Sangjoo Department of Biological Engineering, Inha University, Nam-gu, 402751, Incheon, Republic of Korea aut Morya V. Department of Biological Engineering, Inha University, Nam-gu, 402751, Incheon, Republic of Korea aut Nguyen Dong Department of Biological Engineering, Inha University, Nam-gu, 402751, Incheon, Republic of Korea aut Singh Birendra Department of Biological Engineering, Inha University, Nam-gu, 402751, Incheon, Republic of Korea aut Lee Hyang-Bok Department of Biological Engineering, Inha University, Nam-gu, 402751, Incheon, Republic of Korea aut Kim Eun-Ki Department of Biological Engineering, Inha University, Nam-gu, 402751, Incheon, Republic of Korea aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 403/1-2(2015-05-01), 61-71 0300-8177 403:1-2<61 2015 403 11010 https://doi.org/10.1007/s11010-015-2337-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2337-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Park Sangjoo Department of Biological Engineering, Inha University, Nam-gu, 402751, Incheon, Republic of Korea aut NATIONALLICENCE 700 1- Morya V. Department of Biological Engineering, Inha University, Nam-gu, 402751, Incheon, Republic of Korea aut NATIONALLICENCE 700 1- Nguyen Dong Department of Biological Engineering, Inha University, Nam-gu, 402751, Incheon, Republic of Korea aut NATIONALLICENCE 700 1- Singh Birendra Department of Biological Engineering, Inha University, Nam-gu, 402751, Incheon, Republic of Korea aut NATIONALLICENCE 700 1- Lee Hyang-Bok Department of Biological Engineering, Inha University, Nam-gu, 402751, Incheon, Republic of Korea aut NATIONALLICENCE 700 1- Kim Eun-Ki Department of Biological Engineering, Inha University, Nam-gu, 402751, Incheon, Republic of Korea aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 403/1-2(2015-05-01), 61-71 0300-8177 403:1-2<61 2015 403 11010