caa a22 4500 606236880 CHVBK 20210128101250.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s11010-015-2333-2 doi (NATIONALLICENCE)springer-10.1007/s11010-015-2333-2 Foxp3 regulates ratio of Treg and NKT cells in a mouse model of asthma [Elektronische Daten] [Yanming Lu, Yinshi Guo, Linyun Xu, Yaqin Li, Lanfang Cao] Chronic inflammatory disorder of the airways causes asthma. Regulatory T cells (Treg cells) and Natural killer T cells (NKT cells) both play critical roles in the pathogenesis of asthma. Activation of Treg cells requires Foxp3, whereas whether Foxp3 may regulate the ratio of Treg and NKT cells to affect asthma is uncertain. In an ovalbumin (OVA)-induced mouse model of asthma, we either increased Treg cells by lentivirus-mediated forced expression of exogenous Foxp3, or increased NKT cells by stimulation with its activator α-GalCer. We found that the CD4+CD25+ Treg cells increased by forced Foxp3 expression, and decreased by α-GalCer, while the CD3+CD161+ NKT cells decreased by forced Foxp3 expression, and increased by α-GalCer. Moreover, forced Foxp3 expression, but not α-GalCer, significantly alleviated the hallmarks of asthma. Furthermore, forced Foxp3 increased levels of IL_10 and TGFβ1, and α-GalCer increased levels of IL_4 and INFγ in the OVA-treated lung. Taken together, our study suggests that Foxp3 may activate Treg cells and suppress NKT cells in asthma. Treg and NKT cells may antagonize the effects of each other in asthma. Springer Science+Business Media New York, 2015 OVA nationallicence Asthma nationallicence Regulatory T cells (Treg cells) nationallicence Natural killer T cells (NKT cells) nationallicence Foxp3 nationallicence α-GalCer nationallicence Lu Yanming Department of Pediatrics, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, 145 Shandongzhong Road, 200001, Shanghai, China aut Guo Yinshi Department of Allergy, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, 145 Shandongzhong Road, 200001, Shanghai, China aut Xu Linyun Department of Pediatrics, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, 145 Shandongzhong Road, 200001, Shanghai, China aut Li Yaqin Department of Pediatrics, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, 145 Shandongzhong Road, 200001, Shanghai, China aut Cao Lanfang Department of Pediatrics, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, 145 Shandongzhong Road, 200001, Shanghai, China aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 403/1-2(2015-05-01), 25-31 0300-8177 403:1-2<25 2015 403 11010 https://doi.org/10.1007/s11010-015-2333-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2333-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lu Yanming Department of Pediatrics, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, 145 Shandongzhong Road, 200001, Shanghai, China aut NATIONALLICENCE 700 1- Guo Yinshi Department of Allergy, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, 145 Shandongzhong Road, 200001, Shanghai, China aut NATIONALLICENCE 700 1- Xu Linyun Department of Pediatrics, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, 145 Shandongzhong Road, 200001, Shanghai, China aut NATIONALLICENCE 700 1- Li Yaqin Department of Pediatrics, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, 145 Shandongzhong Road, 200001, Shanghai, China aut NATIONALLICENCE 700 1- Cao Lanfang Department of Pediatrics, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, 145 Shandongzhong Road, 200001, Shanghai, China aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 403/1-2(2015-05-01), 25-31 0300-8177 403:1-2<25 2015 403 11010