caa a22 4500 606236899 CHVBK 20210128101250.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s11010-015-2334-1 doi (NATIONALLICENCE)springer-10.1007/s11010-015-2334-1 MiR-1224-5p acts as a tumor suppressor by targeting CREB1 in malignant gliomas [Elektronische Daten] [Jin Qian, Rui Li, Ying-Yi Wang, Yan Shi, Wen-Kang Luan, Tao Tao, Jun-Xia Zhang, Yi-Chang Xu, Yong-Ping You] The dysregulation of miR-1224-5p has been reported in several human cancers. However, the expression and function of miR-1224-5p in glioma remains unknown. The aim of our study was to investigate the effect of miR-1224-5p on glioma cells and to determine its functional signaling mediators. Using 198 glioma samples within the Chinese Glioma Genome Atlas expression dataset, we demonstrated that miR-1224-5p expression is decreased in high-grade gliomas when compared with low-grade gliomas. Differential miR-1224-5p expression in 50 randomly selected samples was verified by in situ hybridization. The expression of miR-1224-5p was shown to positively correlate with overall survival in 82 glioblastoma patients. Exogenous expression of miR-1224-5p in glioma cells suppressed proliferation and invasion and promoted apoptosis. Target prediction algorithms identified a consensus miR-1224-5p recognition site in the 3′UTR of the cAMP response element-binding protein (CREB1) gene, and this sequence was shown to directly confer miR-1224-5p repression in luciferase reporter assays. Furthermore, exogenous miR-1224-5p expression was shown to down-regulate CREB1, as well as its downstream target genes matrix metalloproteinase-9 and B-cell lymphoma-2. Conversely, over-expression of CREB1 reversed the effect of miR-1224-5p on the proliferation, invasion, and apoptosis of glioma cells. These data indicate that miR-1224-5p may inhibit tumor-associated activity in malignant gliomas by targeting CREB1. Springer Science+Business Media New York, 2015 MiR-1224-5p nationallicence CREB1 nationallicence Glioma nationallicence Cell proliferation nationallicence Invasion nationallicence Apoptosis nationallicence Qian Jin Department of Neurosurgery, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, 210029, Nanjing, China aut Li Rui Department of Neurosurgery, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, 210029, Nanjing, China aut Wang Ying-Yi Department of Neurosurgery, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, 210029, Nanjing, China aut Shi Yan Department of Neurosurgery, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, 210029, Nanjing, China aut Luan Wen-Kang Department of Neurosurgery, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, 210029, Nanjing, China aut Tao Tao Department of Neurosurgery, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, 210029, Nanjing, China aut Zhang Jun-Xia Department of Neurosurgery, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, 210029, Nanjing, China aut Xu Yi-Chang Department of Neurosurgery, The People's Hospital of Xuancheng City, 242000, Xuancheng, China aut You Yong-Ping Department of Neurosurgery, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, 210029, Nanjing, China aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 403/1-2(2015-05-01), 33-41 0300-8177 403:1-2<33 2015 403 11010 https://doi.org/10.1007/s11010-015-2334-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2334-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Qian Jin Department of Neurosurgery, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, 210029, Nanjing, China aut NATIONALLICENCE 700 1- Li Rui Department of Neurosurgery, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, 210029, Nanjing, China aut NATIONALLICENCE 700 1- Wang Ying-Yi Department of Neurosurgery, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, 210029, Nanjing, China aut NATIONALLICENCE 700 1- Shi Yan Department of Neurosurgery, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, 210029, Nanjing, China aut NATIONALLICENCE 700 1- Luan Wen-Kang Department of Neurosurgery, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, 210029, Nanjing, China aut NATIONALLICENCE 700 1- Tao Tao Department of Neurosurgery, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, 210029, Nanjing, China aut NATIONALLICENCE 700 1- Zhang Jun-Xia Department of Neurosurgery, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, 210029, Nanjing, China aut NATIONALLICENCE 700 1- Xu Yi-Chang Department of Neurosurgery, The People's Hospital of Xuancheng City, 242000, Xuancheng, China aut NATIONALLICENCE 700 1- You Yong-Ping Department of Neurosurgery, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, 210029, Nanjing, China aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 403/1-2(2015-05-01), 33-41 0300-8177 403:1-2<33 2015 403 11010