caa a22 4500 606236902 CHVBK 20210128101250.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s11010-015-2338-x doi (NATIONALLICENCE)springer-10.1007/s11010-015-2338-x Advanced glycation end products promote proliferation and suppress autophagy via reduction of Cathepsin D in rat vascular smooth muscle cells [Elektronische Daten] [Mingfeng Ma, Xiaofan Guo, Ye Chang, Chao Li, Xin Meng, Si Li, Zhen-Xian Du, Hua-Qin Wang, Yingxian Sun] Autophagy is closely involved in vascular smooth muscle cell (VSMC) function, but little is known about the association between advanced glycation end products (AGEs) and autophagy and its role in AGEs-induced proliferation and migration of VSMCs. The current study investigated the effects of AGEs on the phenotypic modulation and autophagy of VSMCs, as well as the potential underlying mechanisms. Primary rat VSMCs were treated with bovine serum albumin or AGEs. Cell proliferation was detected by MTT assay, real-time cell analyzer and EdU incorporation. Cell cycle was analyzed by Hoechst staining and flow cytometry. The migration of VSMCs was detected by wound-healing assay and transwell migration assay. LC3 transition and p62 accumulation were detected using Western blotting. Acidic vacuoles were measured using AO and MDC staining. Cathepsin D (CatD) was transduced to VSMCs via lentiviral vectors. AGEs enhanced proliferation and migration of primary rat VSMC in a time-dependent manner. AGEs significantly increased LC3-II transition and p62 expression, as well as accumulation of acidic vacuole, which was not further increased by bafilomycin A1. AGEs decreased CatD expression in a time-dependent pattern, and overexpression of CatD prohibited autophagy attenuation mediated by AGEs. CatD overexpression suppressed AGEs-induced proliferation of VSMCs. Nevertheless, CatD exhibited no effects on AGEs-induced migration of VSMCs. AGEs promote proliferation of VSMCs and suppress autophagy, at least in part via CatD reduction. Springer Science+Business Media New York, 2015 Diabetic vascular complication nationallicence Advanced glycation end products (AGEs) nationallicence Cathepsin D (CatD) nationallicence Autophagy nationallicence Phenotypic modulation nationallicence Vascular smooth muscle cells (VSMCs) nationallicence Ma Mingfeng Department of Cardiology, The First Hospital of China Medical University, 155 Nanjing North Street, Heping District, 110001, Shenyang, Liaoning, People's Republic of China aut Guo Xiaofan Department of Cardiology, The First Hospital of China Medical University, 155 Nanjing North Street, Heping District, 110001, Shenyang, Liaoning, People's Republic of China aut Chang Ye Department of Cardiology, The First Hospital of China Medical University, 155 Nanjing North Street, Heping District, 110001, Shenyang, Liaoning, People's Republic of China aut Li Chao Department of Biochemistry and Molecular Biology, China Medical University, Shenyang, Liaoning, People's Republic of China aut Meng Xin Department of Biochemistry and Molecular Biology, China Medical University, Shenyang, Liaoning, People's Republic of China aut Li Si Department of Endocrinology and Metabolism, The First Hospital of China Medical University, Shenyang, Liaoning, People's Republic of China aut Du Zhen-Xian Department of Endocrinology and Metabolism, The First Hospital of China Medical University, Shenyang, Liaoning, People's Republic of China aut Wang Hua-Qin Department of Biochemistry and Molecular Biology, China Medical University, Shenyang, Liaoning, People's Republic of China aut Sun Yingxian Department of Cardiology, The First Hospital of China Medical University, 155 Nanjing North Street, Heping District, 110001, Shenyang, Liaoning, People's Republic of China aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 403/1-2(2015-05-01), 73-83 0300-8177 403:1-2<73 2015 403 11010 https://doi.org/10.1007/s11010-015-2338-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2338-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ma Mingfeng Department of Cardiology, The First Hospital of China Medical University, 155 Nanjing North Street, Heping District, 110001, Shenyang, Liaoning, People's Republic of China aut NATIONALLICENCE 700 1- Guo Xiaofan Department of Cardiology, The First Hospital of China Medical University, 155 Nanjing North Street, Heping District, 110001, Shenyang, Liaoning, People's Republic of China aut NATIONALLICENCE 700 1- Chang Ye Department of Cardiology, The First Hospital of China Medical University, 155 Nanjing North Street, Heping District, 110001, Shenyang, Liaoning, People's Republic of China aut NATIONALLICENCE 700 1- Li Chao Department of Biochemistry and Molecular Biology, China Medical University, Shenyang, Liaoning, People's Republic of China aut NATIONALLICENCE 700 1- Meng Xin Department of Biochemistry and Molecular Biology, China Medical University, Shenyang, Liaoning, People's Republic of China aut NATIONALLICENCE 700 1- Li Si Department of Endocrinology and Metabolism, The First Hospital of China Medical University, Shenyang, Liaoning, People's Republic of China aut NATIONALLICENCE 700 1- Du Zhen-Xian Department of Endocrinology and Metabolism, The First Hospital of China Medical University, Shenyang, Liaoning, People's Republic of China aut NATIONALLICENCE 700 1- Wang Hua-Qin Department of Biochemistry and Molecular Biology, China Medical University, Shenyang, Liaoning, People's Republic of China aut NATIONALLICENCE 700 1- Sun Yingxian Department of Cardiology, The First Hospital of China Medical University, 155 Nanjing North Street, Heping District, 110001, Shenyang, Liaoning, People's Republic of China aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 403/1-2(2015-05-01), 73-83 0300-8177 403:1-2<73 2015 403 11010