caa a22 4500 606236929 CHVBK 20210128101250.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s11010-015-2343-0 doi (NATIONALLICENCE)springer-10.1007/s11010-015-2343-0 Hypoxic stress and IL-7 gene overexpression enhance the fusion potential of rat bone marrow mesenchymal stem cells with bovine renal epithelial cells [Elektronische Daten] [Rakhshinda Habib, Kanwal Haneef, Nadia Naeem, Irfan Khan, Siddiqua Jamall, Atta-ur-Rahman,, Asmat Salim] Transplantation of mesenchymal stem cells (MSCs) has been shown to enhance the improvement in kidney function following injury. However, the poor survival and grafting of the stem cells to the site of injury has restricted their therapeutic efficacy. Accelerated regeneration potential of MSCs has been observed when they were exposed to hypoxic stress or genetic modulation by various cytokines and growth factors. These preconditioning strategies may stimulate endogenous mechanisms resulting in multiple cellular responses. In this study, we used IL-7 gene to transfect MSCs. IL-7 is a hematopoietic growth factor that plays an important role in cell survival, proliferation, and differentiation. MSCs were also subjected to hypoxic stress for 8 and 24h. These preconditioned MSCs were co-cultured with cisplatin-treated injured Mardin-Darby bovine kidney (MDBK) cells and their fusion potential was analyzed. Flow cytometry of fluorescently labeled preconditioned MSCs and injured MDBK cells revealed evidence of significant (P<0.001) cell fusion compared to that of the normal MSCs. In addition, we also observed improved migration ability of these preconditioned MSCs in the in vitro wound healing assay, as compared to the normal MSCs. We conclude that hypoxic stress and IL-7 overexpression can enhance the renal regeneration potential of MSCs. This study would help in designing more potent therapeutic strategy in which preconditioned MSCs can be used for renal regeneration. Springer Science+Business Media New York, 2015 Acute kidney injury nationallicence Hypoxia nationallicence Co-culture nationallicence Fusion nationallicence Preconditioning nationallicence Habib Rakhshinda Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, 75270, Karachi, Pakistan aut Haneef Kanwal Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, 75270, Karachi, Pakistan aut Naeem Nadia Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, 75270, Karachi, Pakistan aut Khan Irfan Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, 75270, Karachi, Pakistan aut Jamall Siddiqua Department of Biochemistry, University of Karachi, Karachi, Pakistan aut Atta-ur-Rahman, Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, 75270, Karachi, Pakistan aut Salim Asmat Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, 75270, Karachi, Pakistan aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 403/1-2(2015-05-01), 125-137 0300-8177 403:1-2<125 2015 403 11010 https://doi.org/10.1007/s11010-015-2343-0 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2343-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Habib Rakhshinda Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, 75270, Karachi, Pakistan aut NATIONALLICENCE 700 1- Haneef Kanwal Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, 75270, Karachi, Pakistan aut NATIONALLICENCE 700 1- Naeem Nadia Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, 75270, Karachi, Pakistan aut NATIONALLICENCE 700 1- Khan Irfan Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, 75270, Karachi, Pakistan aut NATIONALLICENCE 700 1- Jamall Siddiqua Department of Biochemistry, University of Karachi, Karachi, Pakistan aut NATIONALLICENCE 700 1- Atta-ur-Rahman Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, 75270, Karachi, Pakistan aut NATIONALLICENCE 700 1- Salim Asmat Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, 75270, Karachi, Pakistan aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 403/1-2(2015-05-01), 125-137 0300-8177 403:1-2<125 2015 403 11010