caa a22 4500 606236937 CHVBK 20210128101250.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s11010-015-2335-0 doi (NATIONALLICENCE)springer-10.1007/s11010-015-2335-0 Protective effects of genistein in homocysteine-induced endothelial cell inflammatory injury [Elektronische Daten] [Shengbo Han, Hui Wu, Wenxue Li, Pan Gao] Hyperhomocysteinemia is a risk factor for cardiovascular disease and the mechanism of homocysteine (HCY)-induced vascular endothelial cell injury has been intensively studied for many years. Recently, a large number of studies have shown inhibitory effects of genistein (GEN), a soy isoflavone, in the process of endothelial cell injury. In the present study, the protective effects of GEN in HCY-induced endothelial cell inflammatory injury were investigated. A model of HCY-induced endothelial cell (ECV-304) inflammatory injury was established in vitro, and the protective effect of GEN in this procession was explored. According to our results, GEN protected HCY-induced endothelial cell from viability decreases, meanwhile prevented the changes of cell morphology and the production of reactive oxygen species (ROS). The expression of NF-kB P-65, IL-6, and ICAM-1 was all down-regulated. During the HCY-induced endothelial cell injury, the endothelial cell apoptosis and proliferation disorder were alleviated. Therefore, we conclude that HCY-induced endothelial cell inflammatory injury could be blocked by GEN. The present findings suggest that GEN protects HCY-induced endothelial cell inflammatory injury may through reducing the release of ROS, inhibiting NF-kB activation, down-regulating the expression of cytokine IL-6 and adhesion molecules ICAM-1, avoiding inflammatory cells and platelet adhesion, accordingly, leading to a balance of endothelial cell proliferation and apoptosis. Springer Science+Business Media New York, 2015 Cytokine nationallicence Genistein nationallicence Homocysteine nationallicence Inflammatory injury nationallicence ROS nationallicence Han Shengbo Clinical Laboratory of Zhengzhou Traditional Chinese Medicine Hospital, Wenhuagong Road 65, 450007, Zhengzhou, China aut Wu Hui School of Public Health, Xinxiang Medical University, East of JinSui Road of XinXiang City, 453003, Xinxiang, Henan, China aut Li Wenxue Clinical Laboratory of The Eighth People's Hospital of Zhengzhou City, Ruimin street, 450006, Zhengzhou, China aut Gao Pan Clinical Laboratory of Zhengzhou Traditional Chinese Medicine Hospital, Wenhuagong Road 65, 450007, Zhengzhou, China aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 403/1-2(2015-05-01), 43-49 0300-8177 403:1-2<43 2015 403 11010 https://doi.org/10.1007/s11010-015-2335-0 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2335-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Han Shengbo Clinical Laboratory of Zhengzhou Traditional Chinese Medicine Hospital, Wenhuagong Road 65, 450007, Zhengzhou, China aut NATIONALLICENCE 700 1- Wu Hui School of Public Health, Xinxiang Medical University, East of JinSui Road of XinXiang City, 453003, Xinxiang, Henan, China aut NATIONALLICENCE 700 1- Li Wenxue Clinical Laboratory of The Eighth People's Hospital of Zhengzhou City, Ruimin street, 450006, Zhengzhou, China aut NATIONALLICENCE 700 1- Gao Pan Clinical Laboratory of Zhengzhou Traditional Chinese Medicine Hospital, Wenhuagong Road 65, 450007, Zhengzhou, China aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 403/1-2(2015-05-01), 43-49 0300-8177 403:1-2<43 2015 403 11010