caa a22 4500 606236988 CHVBK 20210128101250.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s11010-014-2296-8 doi (NATIONALLICENCE)springer-10.1007/s11010-014-2296-8 The effects of the modulation of NMDA receptors by homocysteine thiolactone and dizocilpine on cardiodynamics and oxidative stress in isolated rat heart [Elektronische Daten] [Ivan Srejovic, Vladimir Jakovljevic, Vladimir Zivkovic, Nevena Barudzic, Ana Radovanovic, Olivera Stanojlovic, Dragan Djuric] In light of the limited data concerning the role of N-methyl-D-aspartate (NMDA) receptors in cardiac function, the aim of the present study was to determine the role of NMDA receptors in cardiac function, as well as the possible role played by the oxidative stress induced by the overstimulation of NMDA receptors in isolated rat heart. The hearts of male, Wistar albino rats (n=24, 12 in each experimental group, BM 180-200g) were retrogradely perfused at a constant perfusion pressure (70cm H2O), using the Langendorff technique, and cardiodynamic parameters were determined during the subsequent administration of DL-homocysteine thiolactone (DL-Hcy TLHC) alone, the combination of DL-Hcy TLHC and dizocilpine (MK-801), and MK-801 alone. In the second experimental group, the order of the administration of each of the substances was reversed. The oxidative stress biomarkers, including thiobarbituric acid reactive substances (TBARS), NO2 −, O2 − and H2O2, were each determined spectrophotometrically. DL-Hcy TLHC and MK-801 depressed cardiac function. DL-Hcy TLHC decreased oxidative stress, a finding that contrasted with the results of the experiments in which MK-801 was administered first. The findings of this study were suggestive of the likely role played by NMDA receptors in the regulation of cardiac function and coronary circulation in isolated rat heart. Springer Science+Business Media New York, 2014 N-methyl-D-aspartate receptor nationallicence DL-homocysteine thiolactone nationallicence Dizocilpine nationallicence Cardiodynamics nationallicence Oxidative stress nationallicence NMDA : N-methyl-D-aspartate nationallicence DL-Hcy TLHC : DL-homocysteine thiolactone nationallicence AMPA : α-Amino-3-hydrohy-5-methyl-4-izoxazolepropionate nationallicence NR1 : Glycine-binding subunit nationallicence NR2 : Glutamate-binding subunit nationallicence CNS : Central nervous system nationallicence Hcy : Homocysteine nationallicence HHcy : Hyperhomocysteinemia nationallicence MMP-9 : Metalloproteinase-9 nationallicence CF : Coronary flow nationallicence dp/dt max : Maximum rate of pressure development in the left ventricle nationallicence dp/dt mix : Minimum rate of pressure development in the left ventricle nationallicence SLVP : Systolic left ventricular pressure nationallicence DLVP : Diastolic left ventricular pressure nationallicence HR : Heart rate nationallicence TBARS : Thiobarbituric acid reactive substances nationallicence Srejovic Ivan Department of Physiology, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia aut Jakovljevic Vladimir Department of Physiology, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia aut Zivkovic Vladimir Department of Physiology, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia aut Barudzic Nevena Department of Pharmacy, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia aut Radovanovic Ana Department of Pharmacy, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia aut Stanojlovic Olivera Institute of Medical Physiology "Richard Burian”, School of Medicine, University of Belgrade, Belgrade, Serbia aut Djuric Dragan Institute of Medical Physiology "Richard Burian”, School of Medicine, University of Belgrade, Belgrade, Serbia aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 401/1-2(2015-03-01), 97-105 0300-8177 401:1-2<97 2015 401 11010 https://doi.org/10.1007/s11010-014-2296-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-014-2296-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Srejovic Ivan Department of Physiology, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia aut NATIONALLICENCE 700 1- Jakovljevic Vladimir Department of Physiology, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia aut NATIONALLICENCE 700 1- Zivkovic Vladimir Department of Physiology, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia aut NATIONALLICENCE 700 1- Barudzic Nevena Department of Pharmacy, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia aut NATIONALLICENCE 700 1- Radovanovic Ana Department of Pharmacy, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia aut NATIONALLICENCE 700 1- Stanojlovic Olivera Institute of Medical Physiology "Richard Burian”, School of Medicine, University of Belgrade, Belgrade, Serbia aut NATIONALLICENCE 700 1- Djuric Dragan Institute of Medical Physiology "Richard Burian”, School of Medicine, University of Belgrade, Belgrade, Serbia aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 401/1-2(2015-03-01), 97-105 0300-8177 401:1-2<97 2015 401 11010