caa a22 4500 606237003 CHVBK 20210128101250.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s11010-014-2286-x doi (NATIONALLICENCE)springer-10.1007/s11010-014-2286-x Curcumin analog UBS109 prevents bone marrow osteoblastogenesis and osteoclastogenesis disordered by coculture with breast cancer MDA-MB-231 bone metastatic cells in vitro [Elektronische Daten] [Masayoshi Yamaguchi, Shijun Zhu, M. Weitzmann, James Snyder, Mamoru Shoji] UBS109 is a curcumin analog that possessesantitumor properties has been shown to stimulate osteoblastogenesis and suppress osteoclastogenesis in vitro. This study was undertaken to determine whether UBS109 might alleviate the inhibitory activity of breast cancer cells on osteoblastic mineralization and stimulatory effects on osteoclastogenesis. Mouse bone marrow cells werecocultured with breast cancer MDA-MB-231 bone metastatic cells in vitro. UBS109 stimulated osteoblastic mineralization and suppressed adipogenesis and osteoclastogenesis in bone marrow culture. Coculture with MDA-MB-231 cells suppressed osteoblastic mineralization and enhanced osteoclastogenesis in bone marrow culture. Effects that were reserved by UBS109 (50-200nM). Mineralization in preosteoblastic MC3T3-E1 cells was suppressed by coculture with MDA-MB-231 cells, while MDA-MB-231 cells did not have effects on osteoclastogenesis of RAW267.4 cells in vitro. UBS109 (500nM) revealed toxic effects on MDA-MB-231 bone metastatic cells. This study demonstrates that UBS109, which is anantitumor agent, reveals restorative effects on bone marrow cell differentiation disordered by coculture with breast cancer MDA-MB-231 bone metastatic cells in vitro. This in vitro model may be a useful tool to evaluate the mechanism of breast cancer cell bone metastasis. Springer Science+Business Media New York, 2014 UBS109 nationallicence Curcumin analog nationallicence Osteoblastogenesis nationallicence Adipogenesis nationallicence Osteoclastogenesis nationallicence Breast cancer cell nationallicence Bone metastasis nationallicence Yamaguchi Masayoshi Department of Hematology and Medical Oncology, Winship Cancer Institute, Emory University School of Medicine, 1365 C Clifton Road NE, 30322, Atlanta, GA, USA aut Zhu Shijun Department of Hematology and Medical Oncology, Winship Cancer Institute, Emory University School of Medicine, 1365 C Clifton Road NE, 30322, Atlanta, GA, USA aut Weitzmann M. The Atlanta Department of Veterans Affairs Medical Center, Decature, GA, USA aut Snyder James Department of Chemistry, Emory University, 30322, Atlanta, GA, USA aut Shoji Mamoru Department of Hematology and Medical Oncology, Winship Cancer Institute, Emory University School of Medicine, 1365 C Clifton Road NE, 30322, Atlanta, GA, USA aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 401/1-2(2015-03-01), 1-10 0300-8177 401:1-2<1 2015 401 11010 https://doi.org/10.1007/s11010-014-2286-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-014-2286-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yamaguchi Masayoshi Department of Hematology and Medical Oncology, Winship Cancer Institute, Emory University School of Medicine, 1365 C Clifton Road NE, 30322, Atlanta, GA, USA aut NATIONALLICENCE 700 1- Zhu Shijun Department of Hematology and Medical Oncology, Winship Cancer Institute, Emory University School of Medicine, 1365 C Clifton Road NE, 30322, Atlanta, GA, USA aut NATIONALLICENCE 700 1- Weitzmann M. The Atlanta Department of Veterans Affairs Medical Center, Decature, GA, USA aut NATIONALLICENCE 700 1- Snyder James Department of Chemistry, Emory University, 30322, Atlanta, GA, USA aut NATIONALLICENCE 700 1- Shoji Mamoru Department of Hematology and Medical Oncology, Winship Cancer Institute, Emory University School of Medicine, 1365 C Clifton Road NE, 30322, Atlanta, GA, USA aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 401/1-2(2015-03-01), 1-10 0300-8177 401:1-2<1 2015 401 11010