caa a22 4500 606237011 CHVBK 20210128101250.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s11010-014-2302-1 doi (NATIONALLICENCE)springer-10.1007/s11010-014-2302-1 Diminished nitric oxide generation from neutrophils suppresses platelet activation in chronic renal failure [Elektronische Daten] [Daniele Abrantes, Tatiana Brunini, Cristiane Matsuura, Wanda Mury, Carolina Corrêa, Sérgio Santos, Monique Ormonde do Carmo, Antônio Mendes-Ribeiro] Chronic renal failure (CRF) is a complex clinical condition associated with accelerated atherosclerosis and thrombosis leading to cardiovascular events. The aim of this study was to investigate in detail the NO pathway in neutrophils obtained from hemodialysis patients and its association with platelet function and oxidative status. Fifteen CRF patients on hemodialysis and fifteen controls were included in this study. Laboratory and experimental evaluations were performed after hemodialysis in CRF patients. We evaluated l-[3H] arginine transport, NO synthase (NOS) activity, amino acid concentration in neutrophils, and expressions of NOS isoforms and p47phox by western blotting. Platelet aggregation was analyzed in the presence or absence of neutrophils. Oxidative status was measured through glutathione peroxidase, catalase activities, protein oxidation, lipid peroxidation, and DNA/RNA oxidation in serum. Basal NOS activity (pmol/106 cells/min) was impaired in CRF patients on hemodialysis (0.33±0.17) compared to controls (0.65±0.12), whereas the expression of NOS isoforms remained unaltered. l-Arginine transport into neutrophils was similar in CRF patients on hemodialysis and controls. In addition, intracellular concentration of l-arginine was increased fourfold in the patient group. Systemic oxidative stress markers were not affected by CRF. On the other hand, NADPH oxidase subunit p47phox in neutrophils was overexpressed in CRF. In the presence of neutrophils, there was a reduction time-dependent in platelet aggregation in both groups with no difference between them. This data suggest that reduced basal generation of NO by neutrophils in CRF patients on hemodialysis occurs independently of l-arginine bioavailability and is able to suppress platelet activation. Springer Science+Business Media New York, 2014 Nitric oxide nationallicence Neutrophils nationallicence Oxidative stress nationallicence Platelets nationallicence Chronic renal failure nationallicence Abrantes Daniele Department of Pharmacology and Psychobiology, University of the State of Rio de Janeiro, 20551-030, Rio de Janeiro, Brazil aut Brunini Tatiana Department of Pharmacology and Psychobiology, University of the State of Rio de Janeiro, 20551-030, Rio de Janeiro, Brazil aut Matsuura Cristiane Department of Pharmacology and Psychobiology, University of the State of Rio de Janeiro, 20551-030, Rio de Janeiro, Brazil aut Mury Wanda Department of Pharmacology and Psychobiology, University of the State of Rio de Janeiro, 20551-030, Rio de Janeiro, Brazil aut Corrêa Carolina Department of Pharmacology and Psychobiology, University of the State of Rio de Janeiro, 20551-030, Rio de Janeiro, Brazil aut Santos Sérgio Nephrology Discipline, University of the State of Rio de Janeiro, Rio de Janeiro, Brazil aut Ormonde do Carmo Monique Department of Pharmacology and Psychobiology, University of the State of Rio de Janeiro, 20551-030, Rio de Janeiro, Brazil aut Mendes-Ribeiro Antônio Department of Pharmacology and Psychobiology, University of the State of Rio de Janeiro, 20551-030, Rio de Janeiro, Brazil aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 401/1-2(2015-03-01), 147-153 0300-8177 401:1-2<147 2015 401 11010 https://doi.org/10.1007/s11010-014-2302-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-014-2302-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Abrantes Daniele Department of Pharmacology and Psychobiology, University of the State of Rio de Janeiro, 20551-030, Rio de Janeiro, Brazil aut NATIONALLICENCE 700 1- Brunini Tatiana Department of Pharmacology and Psychobiology, University of the State of Rio de Janeiro, 20551-030, Rio de Janeiro, Brazil aut NATIONALLICENCE 700 1- Matsuura Cristiane Department of Pharmacology and Psychobiology, University of the State of Rio de Janeiro, 20551-030, Rio de Janeiro, Brazil aut NATIONALLICENCE 700 1- Mury Wanda Department of Pharmacology and Psychobiology, University of the State of Rio de Janeiro, 20551-030, Rio de Janeiro, Brazil aut NATIONALLICENCE 700 1- Corrêa Carolina Department of Pharmacology and Psychobiology, University of the State of Rio de Janeiro, 20551-030, Rio de Janeiro, Brazil aut NATIONALLICENCE 700 1- Santos Sérgio Nephrology Discipline, University of the State of Rio de Janeiro, Rio de Janeiro, Brazil aut NATIONALLICENCE 700 1- Ormonde do Carmo Monique Department of Pharmacology and Psychobiology, University of the State of Rio de Janeiro, 20551-030, Rio de Janeiro, Brazil aut NATIONALLICENCE 700 1- Mendes-Ribeiro Antônio Department of Pharmacology and Psychobiology, University of the State of Rio de Janeiro, 20551-030, Rio de Janeiro, Brazil aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 401/1-2(2015-03-01), 147-153 0300-8177 401:1-2<147 2015 401 11010