caa a22 4500 606237046 CHVBK 20210128101251.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s11010-014-2303-0 doi (NATIONALLICENCE)springer-10.1007/s11010-014-2303-0 The effect of extended passaging on the phenotype and osteogenic potential of human umbilical cord mesenchymal stem cells [Elektronische Daten] [Zhe Shi, Liang Zhao, Gengtao Qiu, Ruixuan He, Michael Detamore] Retaining biological characteristics in the extended passaging is crucial for human umbilical cord mesenchymal stem cells (hUCMSCs) in tissue engineering. We aimed to assess morphology, viability, MSC marker expression, and osteogenic activity of hUCSMCs after extended passaging. Passages 4 (P4) and 16 (P16) hUCMSCs displayed similar morphology and viability. The flow cytometry results showed that CD73, CD90, and CD105 were highly expressed at P1-P16. CD166 expression decreased progressively from 90% at P2 to 61.5% at P5 (p<0.05), followed by stable expression through P16. Results from calcium deposition alkaline phosphatase activity and RT-PCR assay showed that both P4 and P16 hUCMSCs differentiated down an osteogenic lineage, with no significant difference in osteogenic capacity (p<0.05). High-passage UMCSCs maintained stable expression of MSC CD markers as well as stable osteogenic activity. hUCMSCs may thus be suitable for tissue engineering and regenerative medicine applications. Springer Science+Business Media New York, 2015 Mesenchymal stem cell nationallicence Human umbilical cord nationallicence Proliferation nationallicence Osteogenic differentiation nationallicence CD166 nationallicence Shi Zhe Department of Orthopaedic Surgery, The Third Affilliated Hospital, Southern Medical University, Guangzhou, China aut Zhao Liang Department of Orthopaedic Surgery, Nanfang Hospital, Southern Medical University, 1838 North Guangzhou Ave, 510515, Guangzhou, China aut Qiu Gengtao Department of Orthopaedic Surgery, Nanfang Hospital, Southern Medical University, 1838 North Guangzhou Ave, 510515, Guangzhou, China aut He Ruixuan Department of Orthopaedic Surgery, Nanfang Hospital, Southern Medical University, 1838 North Guangzhou Ave, 510515, Guangzhou, China aut Detamore Michael Department of Chemical & Petroleum Engineering, University of Kansas, 4132 Learned Hall, 1530 W 15th Street, 66045, Lawrence, KS, USA aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 401/1-2(2015-03-01), 155-164 0300-8177 401:1-2<155 2015 401 11010 https://doi.org/10.1007/s11010-014-2303-0 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-014-2303-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shi Zhe Department of Orthopaedic Surgery, The Third Affilliated Hospital, Southern Medical University, Guangzhou, China aut NATIONALLICENCE 700 1- Zhao Liang Department of Orthopaedic Surgery, Nanfang Hospital, Southern Medical University, 1838 North Guangzhou Ave, 510515, Guangzhou, China aut NATIONALLICENCE 700 1- Qiu Gengtao Department of Orthopaedic Surgery, Nanfang Hospital, Southern Medical University, 1838 North Guangzhou Ave, 510515, Guangzhou, China aut NATIONALLICENCE 700 1- He Ruixuan Department of Orthopaedic Surgery, Nanfang Hospital, Southern Medical University, 1838 North Guangzhou Ave, 510515, Guangzhou, China aut NATIONALLICENCE 700 1- Detamore Michael Department of Chemical & Petroleum Engineering, University of Kansas, 4132 Learned Hall, 1530 W 15th Street, 66045, Lawrence, KS, USA aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 401/1-2(2015-03-01), 155-164 0300-8177 401:1-2<155 2015 401 11010