caa a22 4500 606237062 CHVBK 20210128101251.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s11010-014-2300-3 doi (NATIONALLICENCE)springer-10.1007/s11010-014-2300-3 Proteomic analysis of the extracellular matrix in idiopathic pes equinovarus [Elektronische Daten] [Martin Ošt'ádal, Adam Eckhardt, Jan Herget, Ivan Mikšík, Pavel Dungl, Jiří Chomiak, Monika Frydrychová, Michal Burian] Idiopathic pes equinovarus is a congenital deformity of the foot and lower leg defined as a fixation of the foot in adduction, supination, and varus. Although the pathogenesis of clubfoot remains unclear, it has been suggested that fibroblasts and growth factors are involved. To directly analyze the protein composition of the extracellular matrix in contracted tissue of patients with clubfoot. A total of 13 infants with idiopathic clubfoot treated with the Ponseti method were included in the present study. Tissue samples were obtained from patients undergoing surgery for relapsed clubfeet. Contracted tissues were obtained from the medial aspect of the talonavicular joint. Protein was extracted after digestion and delipidation using zip-tip C18. Individual collagenous fractions were detected using a chemiluminescent assay. Amino acid analysis of tissue samples revealed a predominance of collagens, namely collagen types I, III, and VI. The high content of glycine and h-proline suggests a predominance of collagens I and III. A total of 19 extracellular matrix proteins were identified. The major result of the present study was the observation that the extracellular matrix in clubfoot is composed of an additional 16 proteins, including collagens V, VI, and XII, as well as the previously described collagen types I and III and transforming growth factor β. The characterization of the general protein composition of the extracellular matrix in various regions of clubfoot may help in understanding the pathogenesis of this anomaly and, thus, contribute to the development of more efficacious therapeutic approaches. Springer Science+Business Media New York, 2014 Collagens nationallicence Extracellular matrix nationallicence Pes equinovarus nationallicence Proteomics nationallicence Ošt'ádal Martin Department of Orthopaedics, 1st Faculty of Medicine, University Hospital Bulovka, Charles University, Budínova 2, 180 00, Prague 8, Czech Republic aut Eckhardt Adam Institute of Physiology, Academy of Sciences of the Czech Republic, Prague 4, Czech Republic aut Herget Jan Institute of Physiology, 2nd Faculty of Medicine, Charles University, Prague 5, Czech Republic aut Mikšík Ivan Institute of Physiology, Academy of Sciences of the Czech Republic, Prague 4, Czech Republic aut Dungl Pavel Department of Orthopaedics, 1st Faculty of Medicine, University Hospital Bulovka, Charles University, Budínova 2, 180 00, Prague 8, Czech Republic aut Chomiak Jiří Department of Orthopaedics, 1st Faculty of Medicine, University Hospital Bulovka, Charles University, Budínova 2, 180 00, Prague 8, Czech Republic aut Frydrychová Monika Department of Orthopaedics, 1st Faculty of Medicine, University Hospital Bulovka, Charles University, Budínova 2, 180 00, Prague 8, Czech Republic aut Burian Michal Department of Orthopaedics, 1st Faculty of Medicine, University Hospital Bulovka, Charles University, Budínova 2, 180 00, Prague 8, Czech Republic aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 401/1-2(2015-03-01), 133-139 0300-8177 401:1-2<133 2015 401 11010 https://doi.org/10.1007/s11010-014-2300-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-014-2300-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ošt'ádal Martin Department of Orthopaedics, 1st Faculty of Medicine, University Hospital Bulovka, Charles University, Budínova 2, 180 00, Prague 8, Czech Republic aut NATIONALLICENCE 700 1- Eckhardt Adam Institute of Physiology, Academy of Sciences of the Czech Republic, Prague 4, Czech Republic aut NATIONALLICENCE 700 1- Herget Jan Institute of Physiology, 2nd Faculty of Medicine, Charles University, Prague 5, Czech Republic aut NATIONALLICENCE 700 1- Mikšík Ivan Institute of Physiology, Academy of Sciences of the Czech Republic, Prague 4, Czech Republic aut NATIONALLICENCE 700 1- Dungl Pavel Department of Orthopaedics, 1st Faculty of Medicine, University Hospital Bulovka, Charles University, Budínova 2, 180 00, Prague 8, Czech Republic aut NATIONALLICENCE 700 1- Chomiak Jiří Department of Orthopaedics, 1st Faculty of Medicine, University Hospital Bulovka, Charles University, Budínova 2, 180 00, Prague 8, Czech Republic aut NATIONALLICENCE 700 1- Frydrychová Monika Department of Orthopaedics, 1st Faculty of Medicine, University Hospital Bulovka, Charles University, Budínova 2, 180 00, Prague 8, Czech Republic aut NATIONALLICENCE 700 1- Burian Michal Department of Orthopaedics, 1st Faculty of Medicine, University Hospital Bulovka, Charles University, Budínova 2, 180 00, Prague 8, Czech Republic aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 401/1-2(2015-03-01), 133-139 0300-8177 401:1-2<133 2015 401 11010