caa a22 4500 606237097 CHVBK 20210128101251.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s11010-014-2287-9 doi (NATIONALLICENCE)springer-10.1007/s11010-014-2287-9 The interplay between glucose and fatty acids on tube formation and fatty acid uptake in the first trimester trophoblast cells, HTR8/SVneo [Elektronische Daten] [Sanjay Basak, Mrinal Das, Vilasagar Srinivas, Asim Duttaroy] The study aims to investigate the impacts of hyperglycemia in the presence of fatty acids on early placentation processes that involves tube formation, cellular growth and proliferation, and metabolic activities of the first trimester trophoblast cells. Effects of maternal circulatory glucose levels that mimic physiological (5.5mM), pre-diabetic (11mM) and diabetic (≥25mM) phenotypes on tube formation (as a measure of angiogenesis in vitro), cellular viability and proliferation, fatty acid uptake and expression of genes associated with invasion, angiogenesis and fatty acid metabolism were examined using HTR8/SVneo cells. Glucose (25mM) induced tube formation, viability, and proliferation of the first trimester trophoblast cells, HTR8/SVneo. Tube formation was, however, disintegrated in the presence of high glucose (40mM) which was partially protected by eicosapentaenoic acid, 20:5n-3 (EPA) and docosahexaenoic acid, 22:6n-3 in vitro. Glucose (25mM)-mediated induction in tube formation was favored by increased cellular uptake of [14C]EPA (p<0.05). Treatment of HTR8/SVneo cells with glucose (25mM) significantly increased mRNA and protein level of matrix metalloproteinase-9 (MMP9) (p<0.05). In addition, glucose (25mM) stimulated the expression of fatty acid binding protein-4, FABP4, and plasma membrane fatty acid binding protein, FABPpm, in these cells (p<0.05). Glucose-stimulated tube formation in a ‘concentration-dependent' manner, and this may involve activation of several factors that include MMP9 and fatty acid uptake and metabolism. Springer Science+Business Media New York, 2014 Glucose nationallicence Tube formation nationallicence Fatty acid uptake nationallicence First trimester trophoblast nationallicence MMP9 nationallicence Docosahexaenoic acid nationallicence FABP4 nationallicence HTR8/SVneo nationallicence Basak Sanjay Department of Nutrition, Institute for Basic Medical Sciences, University of Oslo, Blindern, POB 1046, 0316, Oslo, Norway aut Das Mrinal Department of Nutrition, Institute for Basic Medical Sciences, University of Oslo, Blindern, POB 1046, 0316, Oslo, Norway aut Srinivas Vilasagar Molecular Biology Division, National Institute Nutrition, Indian Council of Medical Research, 500007, Hyderabad, India aut Duttaroy Asim Department of Nutrition, Institute for Basic Medical Sciences, University of Oslo, Blindern, POB 1046, 0316, Oslo, Norway aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 401/1-2(2015-03-01), 11-19 0300-8177 401:1-2<11 2015 401 11010 https://doi.org/10.1007/s11010-014-2287-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-014-2287-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Basak Sanjay Department of Nutrition, Institute for Basic Medical Sciences, University of Oslo, Blindern, POB 1046, 0316, Oslo, Norway aut NATIONALLICENCE 700 1- Das Mrinal Department of Nutrition, Institute for Basic Medical Sciences, University of Oslo, Blindern, POB 1046, 0316, Oslo, Norway aut NATIONALLICENCE 700 1- Srinivas Vilasagar Molecular Biology Division, National Institute Nutrition, Indian Council of Medical Research, 500007, Hyderabad, India aut NATIONALLICENCE 700 1- Duttaroy Asim Department of Nutrition, Institute for Basic Medical Sciences, University of Oslo, Blindern, POB 1046, 0316, Oslo, Norway aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 401/1-2(2015-03-01), 11-19 0300-8177 401:1-2<11 2015 401 11010