caa a22 4500 606237119 CHVBK 20210128101251.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s11010-014-2294-x doi (NATIONALLICENCE)springer-10.1007/s11010-014-2294-x The mechanism of UVB irradiation induced-apoptosis in cataract [Elektronische Daten] [Yinghong Ji, Lei Cai, Tianyu Zheng, Hongfei Ye, Xianfang Rong, Jun Rao, Yi Lu] Cataract is the most common eye disease that causes blindness in patients. Ultraviolet B (UVB) irradiation is considered an important factor leading to cataract by inducing apoptosis in human lens epithelial cells (HLECs), but the mechanism is currently unclear. In this study, we investigated HLECs under different intensities of UVB irradiation and different exposure time. The annexin V-FITC/propidium iodide staining results showed that UVB irradiation could efficiently lead to HLECs apoptosis in time- and dose-dependent manner. The expression of pro-apoptotic Bax gene was promoted by UVB irradiation, while anti-apoptotic Bcl-2 gene expression was inhibited at both transcript and protein levels. Notably, the ratio of Bax/Bcl-2 displayed a high and positive correlation to the proportion of apoptotic HLECs. Mitochondrial dysfunction was also observed with rapid loss of potential (∆Ψ m), as well as changes of the levels of reactive oxygen species, malondialdehyde, total antioxidative capabilities, and superoxide dismutase. In caspase pathway, the level of caspase-3 protein increased after UVB irradiation. All these discovered changes may play important roles in UVB-induced HLECs apoptosis, and would be helpful in understanding the mechanism of UVB-induced cataract and providing potential prevention and treatment strategies. Springer Science+Business Media New York, 2014 UVB irradiation nationallicence Cataract nationallicence Apoptosis nationallicence Caspase-3 nationallicence Human lens epithelial cells (HLECs) nationallicence Ji Yinghong Department of Ophthalmology, Eye & ENT Hospital of Fudan University, No. 83 Fenyang Road, 200031, Shanghai, China aut Cai Lei Department of Ophthalmology, Eye & ENT Hospital of Fudan University, No. 83 Fenyang Road, 200031, Shanghai, China aut Zheng Tianyu Department of Ophthalmology, Eye & ENT Hospital of Fudan University, No. 83 Fenyang Road, 200031, Shanghai, China aut Ye Hongfei Department of Ophthalmology, Eye & ENT Hospital of Fudan University, No. 83 Fenyang Road, 200031, Shanghai, China aut Rong Xianfang Department of Ophthalmology, Eye & ENT Hospital of Fudan University, No. 83 Fenyang Road, 200031, Shanghai, China aut Rao Jun Jiangxi Provincial Cancer Hospital, No. 519 East Beijing Road, 330029, Nanchang, China aut Lu Yi Department of Ophthalmology, Eye & ENT Hospital of Fudan University, No. 83 Fenyang Road, 200031, Shanghai, China aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 401/1-2(2015-03-01), 87-95 0300-8177 401:1-2<87 2015 401 11010 https://doi.org/10.1007/s11010-014-2294-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-014-2294-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ji Yinghong Department of Ophthalmology, Eye & ENT Hospital of Fudan University, No. 83 Fenyang Road, 200031, Shanghai, China aut NATIONALLICENCE 700 1- Cai Lei Department of Ophthalmology, Eye & ENT Hospital of Fudan University, No. 83 Fenyang Road, 200031, Shanghai, China aut NATIONALLICENCE 700 1- Zheng Tianyu Department of Ophthalmology, Eye & ENT Hospital of Fudan University, No. 83 Fenyang Road, 200031, Shanghai, China aut NATIONALLICENCE 700 1- Ye Hongfei Department of Ophthalmology, Eye & ENT Hospital of Fudan University, No. 83 Fenyang Road, 200031, Shanghai, China aut NATIONALLICENCE 700 1- Rong Xianfang Department of Ophthalmology, Eye & ENT Hospital of Fudan University, No. 83 Fenyang Road, 200031, Shanghai, China aut NATIONALLICENCE 700 1- Rao Jun Jiangxi Provincial Cancer Hospital, No. 519 East Beijing Road, 330029, Nanchang, China aut NATIONALLICENCE 700 1- Lu Yi Department of Ophthalmology, Eye & ENT Hospital of Fudan University, No. 83 Fenyang Road, 200031, Shanghai, China aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 401/1-2(2015-03-01), 87-95 0300-8177 401:1-2<87 2015 401 11010