caa a22 4500 606237135 CHVBK 20210128101251.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s11010-014-2301-2 doi (NATIONALLICENCE)springer-10.1007/s11010-014-2301-2 Non-esterified fatty acids promote expression and secretion of angiopoietin-like protein 4 in calf hepatocytes cultured in vitro [Elektronische Daten] [Jianguo Wang, Xiaoyan Zhu, Zhe Wang, Junhu Yao, Baoyu Zhao, Guowen Liu] An elevated plasma non-esterified fatty acids (NEFA) concentration is an important indicator of negative energy balance, which is often associated with metabolic diseases such as fatty liver and ketosis. Angiopoietin-like protein 4 (ANGPTL4) is a hepatokine that plays important roles in the regulation of lipid metabolism. However, the direct effects of high concentrations of NEFA on ANGPTL4 expression and secretion in hepatocytes from cows are not entirely clear. The objective of this study was to investigate the effects of different NEFA concentrations on ANGPTL4 expression and secretion in calf hepatocytes cultured in vitro. NEFA was added to the culture solution at final concentrations of 0.6, 1.2, 1.8, and 2.4mmol/L. After 24h of continuous culture, ANGPTL4 mRNA and protein expression levels in the hepatocytes were determined by real-time polymerase chain reaction and western blot, respectively. ANGPTL4 secretion in the supernatant was determined by enzyme-linked immunosorbent assay. The results showed similar levels of ANGPTL4 expression and secretion following treatment with 0.6 and 1.2mmol/L NEFA, which were higher than those observed for the controls (P<0.05). ANGPTL4 expression and secretion were also similar at 1.8 and 2.4mmol/L NEFA and significantly higher than those observed for controls (P<0.01). These data suggest that high concentrations of NEFA significantly promote ANGPTL4 expression and secretion in bovine hepatocytes. In particular, this promotion occurs in a dose-dependent manner and may be involved in the pathological processes of energy metabolism disorders of dairy cows in the peripartum period. Springer Science+Business Media New York, 2014 Non-esterified fatty acids nationallicence Angiopoietin-like protein 4 nationallicence Dairy cows nationallicence Hepatocytes nationallicence Wang Jianguo College of Veterinary Medicine, Northwest A&F University, 712100, Yangling, Shaanxi, China aut Zhu Xiaoyan UMR 788 Inserm and University Paris-Sud, Kremlin-Bicêtre, France aut Wang Zhe College of Veterinary Medicine, Jilin University, 130062, Changchun, Jilin, China aut Yao Junhu College of Animal Science and Technology, Northwest A&F University, 712100, Yangling, Shaanxi, China aut Zhao Baoyu College of Veterinary Medicine, Northwest A&F University, 712100, Yangling, Shaanxi, China aut Liu Guowen College of Veterinary Medicine, Jilin University, 130062, Changchun, Jilin, China aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 401/1-2(2015-03-01), 141-146 0300-8177 401:1-2<141 2015 401 11010 https://doi.org/10.1007/s11010-014-2301-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 brief-communication jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-014-2301-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Jianguo College of Veterinary Medicine, Northwest A&F University, 712100, Yangling, Shaanxi, China aut NATIONALLICENCE 700 1- Zhu Xiaoyan UMR 788 Inserm and University Paris-Sud, Kremlin-Bicêtre, France aut NATIONALLICENCE 700 1- Wang Zhe College of Veterinary Medicine, Jilin University, 130062, Changchun, Jilin, China aut NATIONALLICENCE 700 1- Yao Junhu College of Animal Science and Technology, Northwest A&F University, 712100, Yangling, Shaanxi, China aut NATIONALLICENCE 700 1- Zhao Baoyu College of Veterinary Medicine, Northwest A&F University, 712100, Yangling, Shaanxi, China aut NATIONALLICENCE 700 1- Liu Guowen College of Veterinary Medicine, Jilin University, 130062, Changchun, Jilin, China aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 401/1-2(2015-03-01), 141-146 0300-8177 401:1-2<141 2015 401 11010