caa a22 4500 606237143 CHVBK 20210128101251.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s11010-014-2307-9 doi (NATIONALLICENCE)springer-10.1007/s11010-014-2307-9 Abarikwu Sunny Department of Biochemistry, University of Port Harcourt, East-West Road, Choba, Nigeria aut Anti-inflammatory effects of kolaviron modulate the expressions of inflammatory marker genes, inhibit transcription factors ERK1/2, p-JNK, NF-κB, and activate Akt expressions in the 93RS2 Sertoli cell lines [Elektronische Daten] [Sunny Abarikwu] The anti-inflammatory effects of kolaviron (Kol-v) have been demonstrated in several experimental models. The ability of Kol-v to modulate the expressions of inflammatory genes in lipopolysaccharide (LPS)-stimulated Sertoli cell line, 93RS2 was investigated in this study. Kol-v decreased the expressions of inflammatory genes TNF-α, Tlr-4, and Nfκb1 and has synergistic effect on LPS-induced COX-2 and iNOS expressions at high concentrations (25-100μM). At lower concentrations (5-15µM), the expressions of TNF-α, IL-6, and IL-1α were down-regulated by Kol-v except Tgfβ1 that was up-regulated. The LPS-induced decrease in the expression of the anti-inflammatory genes IL-3, IL-4, and IL-10 was blocked by Kol-v at all concentrations of Kol-v tested. The LPS-induced phosphorylations of mitogen-activated protein kinase family members (ERK1/2, and p-JNK), decreased IκBα expression, and decreased Akt phosphorylation was blocked by Kol-v. Our results highlight the potential for Kol-v at lower concentration to ameliorate cellular damage caused by local inflammation. Springer Science+Business Media New York, 2014 Kolaviron nationallicence Inflammation nationallicence LPS nationallicence 93RS2 Sertoli cells nationallicence Gene expressions nationallicence MAP-kinase nationallicence Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 401/1-2(2015-03-01), 197-208 0300-8177 401:1-2<197 2015 401 11010 https://doi.org/10.1007/s11010-014-2307-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-014-2307-9 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Abarikwu Sunny Department of Biochemistry, University of Port Harcourt, East-West Road, Choba, Nigeria aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 401/1-2(2015-03-01), 197-208 0300-8177 401:1-2<197 2015 401 11010