caa a22 4500 606237151 CHVBK 20210128101251.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s11010-014-2288-8 doi (NATIONALLICENCE)springer-10.1007/s11010-014-2288-8 Constitutive L-Sox5 overexpression delays differentiation of ATDC5 cells into chondrocytes and correlates with reduced expression of differentiation markers [Elektronische Daten] [Dongmei Sun, Vishwa Deepak, Ping Mu, Haiying Jiang, Xiuming Shi, Zhongbo Liu, Xianlu Zeng, Wenguang Liu] L-Sox5 is a member of sex-determining region Y-type high mobility group box (SOX) family of transcription factors. We assessed the effects of retroviral overexpression of L-Sox5 on chondrocyte differentiation using the clonal murine cell line ATDC5. We observed a temporal-restricted expression pattern of L-Sox5 in insulin-induced ATDC5 cells differentiating toward chondrocyte lineage. The protein expression levels of L-Sox5 showed a drastic decrease in contrast to unaltered mRNA levels during differentiation. L-Sox5 delayed the differentiation of ATDC5 cells as evidenced by Alcian blue staining for proteoglycan synthesis. The mRNA levels of chondrocyte and hypertrophic/osteoarthritic markers were markedly decreased or delayed in L-Sox5 overexpressing cells. L-Sox5 abrogated the promoter activity of Runx2. These results suggest that L-Sox5 protein expression may diminish along with the progress of chondrogenic differentiation. L-Sox5 may act as a negative regulator if expressed aberrantly at least in part by regulating the critical fate of chondrogenesis. Springer Science+Business Media New York, 2014 L-Sox5 nationallicence Chondrogenesis nationallicence Runx2 nationallicence Hypertrophy nationallicence Asporin nationallicence Osteoarthritis nationallicence Sun Dongmei Key Laboratory of Molecular Epigenetics of Ministry of Education of China, Northeast Normal University, Renmin Street 5268, 130024, Changchun, Jilin, China aut Deepak Vishwa Department of Physiology, Faculty of Health Sciences, School of Medicine, University of Pretoria, Private Bag X323, Arcadia, 0007, Pretoria, South Africa aut Mu Ping Key Laboratory of Molecular Epigenetics of Ministry of Education of China, Northeast Normal University, Renmin Street 5268, 130024, Changchun, Jilin, China aut Jiang Haiying Key Laboratory of Molecular Epigenetics of Ministry of Education of China, Northeast Normal University, Renmin Street 5268, 130024, Changchun, Jilin, China aut Shi Xiuming Key Laboratory of Molecular Epigenetics of Ministry of Education of China, Northeast Normal University, Renmin Street 5268, 130024, Changchun, Jilin, China aut Liu Zhongbo Key Laboratory of Molecular Epigenetics of Ministry of Education of China, Northeast Normal University, Renmin Street 5268, 130024, Changchun, Jilin, China aut Zeng Xianlu Key Laboratory of Molecular Epigenetics of Ministry of Education of China, Northeast Normal University, Renmin Street 5268, 130024, Changchun, Jilin, China aut Liu Wenguang Key Laboratory of Molecular Epigenetics of Ministry of Education of China, Northeast Normal University, Renmin Street 5268, 130024, Changchun, Jilin, China aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 401/1-2(2015-03-01), 21-26 0300-8177 401:1-2<21 2015 401 11010 https://doi.org/10.1007/s11010-014-2288-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 brief-communication jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-014-2288-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sun Dongmei Key Laboratory of Molecular Epigenetics of Ministry of Education of China, Northeast Normal University, Renmin Street 5268, 130024, Changchun, Jilin, China aut NATIONALLICENCE 700 1- Deepak Vishwa Department of Physiology, Faculty of Health Sciences, School of Medicine, University of Pretoria, Private Bag X323, Arcadia, 0007, Pretoria, South Africa aut NATIONALLICENCE 700 1- Mu Ping Key Laboratory of Molecular Epigenetics of Ministry of Education of China, Northeast Normal University, Renmin Street 5268, 130024, Changchun, Jilin, China aut NATIONALLICENCE 700 1- Jiang Haiying Key Laboratory of Molecular Epigenetics of Ministry of Education of China, Northeast Normal University, Renmin Street 5268, 130024, Changchun, Jilin, China aut NATIONALLICENCE 700 1- Shi Xiuming Key Laboratory of Molecular Epigenetics of Ministry of Education of China, Northeast Normal University, Renmin Street 5268, 130024, Changchun, Jilin, China aut NATIONALLICENCE 700 1- Liu Zhongbo Key Laboratory of Molecular Epigenetics of Ministry of Education of China, Northeast Normal University, Renmin Street 5268, 130024, Changchun, Jilin, China aut NATIONALLICENCE 700 1- Zeng Xianlu Key Laboratory of Molecular Epigenetics of Ministry of Education of China, Northeast Normal University, Renmin Street 5268, 130024, Changchun, Jilin, China aut NATIONALLICENCE 700 1- Liu Wenguang Key Laboratory of Molecular Epigenetics of Ministry of Education of China, Northeast Normal University, Renmin Street 5268, 130024, Changchun, Jilin, China aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 401/1-2(2015-03-01), 21-26 0300-8177 401:1-2<21 2015 401 11010