caa a22 4500 606240209 CHVBK 20210128101307.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s00508-015-0853-0 doi (NATIONALLICENCE)springer-10.1007/s00508-015-0853-0 Evaluation of the immunochromatographic (Leptocheck) test for detection of specific antibodies against leptospires [Elektronische Daten] [Daša Podgoršek, Tjaša Cerar, Mateja Logar, Gorazd Lešničar, Tatjana Remec, Zvonko Baklan, Emil Pal, Eva Ružić-Sabljić] Summary: Background: Leptospirosis is a febrile worldwide zoonosis. Routine diagnosis of leptospiral infection is based on demonstration of specific antibodies with serological tests. Performance of the reference serological test, the microscopic agglutination test (MAT), requires significant expertise. The aim of our study was to find out if leptospiral infection can be proven with simple, rapid, commercially available immunochromatographic Leptocheck test in order to introduce it for the first level diagnosis in emergency cases with less specialized laboratory staff. Methods: In all, 590 serum samples of patients with clinical manifestations suggestive of leptospirosis were collected and tested with MAT and Leptocheck test. For confirmation of the results some other diagnostic methods such as polymerase chain reaction (PCR) and Leptospira isolation were performed. Results: Results of both serological tests were consistent in 576/590 (97.63 %) cases but Leptocheck gave more positive results in comparison to MAT (36 and 12, respectively) at first patient's testing. Following up the patient, MAT became positive in majority of Leptocheck positive patients at first visit. Leptospiral DNA was detected in nine blood and six urine samples belonging to thirteen different patients while only two samples were culture positive. Conclusion: In comparison with serological tests, PCR and culture have low sensitivity. According to our findings we conclude that Leptocheck test can prove leptospiral infection and could be used for rapid diagnosis of leptospirosis, later the sample should be confirmed with MAT. Springer-Verlag Wien, 2015 Leptospirosis nationallicence Diagnostics nationallicence Serological tests nationallicence Microagglutination test nationallicence Leptocheck test nationallicence Podgoršek Daša Faculty of Medicine Ljubljana, Institute of Microbiology and Immunology, Zaloška 4, 1000, Ljubljana, Slovenia aut Cerar Tjaša Faculty of Medicine Ljubljana, Institute of Microbiology and Immunology, Zaloška 4, 1000, Ljubljana, Slovenia aut Logar Mateja Department of Infectious Diseases, University Medical Center Ljubljana, Zaloška 2, 1000, Ljubljana, Slovenia aut Lešničar Gorazd Department of Infectious Diseases, General Hospital Celje, Oblakova 5, 3000, Celje, Slovenia aut Remec Tatjana Department of Infectious Diseases, General Hospital Novo mesto, Šmihelska cesta 1, 8000, Novo mesto, Slovenia aut Baklan Zvonko Department of Infectious Diseases, University Medical Center Maribor, Ljubljanska ulica 5, 2000, Maribor, Slovenia aut Pal Emil Department of Infectious Diseases, General Hospital Murska Sobota, Ulica dr. Vrbnjaka 6, 9000, Murska Sobota, Slovenia aut Ružić-Sabljić Eva Faculty of Medicine Ljubljana, Institute of Microbiology and Immunology, Zaloška 4, 1000, Ljubljana, Slovenia aut Wiener klinische Wochenschrift Springer Vienna 127/23-24(2015-12-01), 948-953 0043-5325 127:23-24<948 2015 127 508 https://doi.org/10.1007/s00508-015-0853-0 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00508-015-0853-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Podgoršek Daša Faculty of Medicine Ljubljana, Institute of Microbiology and Immunology, Zaloška 4, 1000, Ljubljana, Slovenia aut NATIONALLICENCE 700 1- Cerar Tjaša Faculty of Medicine Ljubljana, Institute of Microbiology and Immunology, Zaloška 4, 1000, Ljubljana, Slovenia aut NATIONALLICENCE 700 1- Logar Mateja Department of Infectious Diseases, University Medical Center Ljubljana, Zaloška 2, 1000, Ljubljana, Slovenia aut NATIONALLICENCE 700 1- Lešničar Gorazd Department of Infectious Diseases, General Hospital Celje, Oblakova 5, 3000, Celje, Slovenia aut NATIONALLICENCE 700 1- Remec Tatjana Department of Infectious Diseases, General Hospital Novo mesto, Šmihelska cesta 1, 8000, Novo mesto, Slovenia aut NATIONALLICENCE 700 1- Baklan Zvonko Department of Infectious Diseases, University Medical Center Maribor, Ljubljanska ulica 5, 2000, Maribor, Slovenia aut NATIONALLICENCE 700 1- Pal Emil Department of Infectious Diseases, General Hospital Murska Sobota, Ulica dr. Vrbnjaka 6, 9000, Murska Sobota, Slovenia aut NATIONALLICENCE 700 1- Ružić-Sabljić Eva Faculty of Medicine Ljubljana, Institute of Microbiology and Immunology, Zaloška 4, 1000, Ljubljana, Slovenia aut NATIONALLICENCE 773 0- Wiener klinische Wochenschrift Springer Vienna 127/23-24(2015-12-01), 948-953 0043-5325 127:23-24<948 2015 127 508