caa a22 4500 606240551 CHVBK 20210128101308.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s00508-015-0727-5 doi (NATIONALLICENCE)springer-10.1007/s00508-015-0727-5 High-frequency oscillatory ventilation combined with partial liquid ventilation in experimental lung injury: effects on lung cell apoptosis [Elektronische Daten] [Huimin Zuo, Linxiang Zeng, Guanghua Guo, Huihong Zeng] Summary: Objective: To investigate the effects of high-frequency oscillatory ventilation (HFOV) and partial liquid ventilation (PLV) on apoptosis of lung tissue induced by steam inhalation injury in rabbit. Design: A prospective, randomized, controlled, multiple-group study. Setting: An animal research laboratory centre in a university burns centre. Subjects: New Zealand rabbits (n=32; 2.25±0.25kg) of either sex. Interventions: The animals were ventilated by HFOV with a mean airway pressure of 10cm H2O, a frequency of 10Hz, an amplitude of 20cm H2O, an inspiratory:expiratory ratio of 1:1, and an FiO2 of 1.0. After the induction of acute lung injury (ALI) by steam inhalation, the animals were randomly divided into four groups: CMV, HFOV, CMV + PLV, HFOV + PLV group. Then they were ventilated for 4h by CMV, HFOV, CMV + PLV and HFOV + PLV, respectively. After the experimental period, cell apoptosis and apoptosis indexes in the lung tissue were assessed with TUNEL FragELTM (Fragment End Labeling). Results: Lung tissue apoptosis indexes in HFOV group and HFOV + PLV group were lower than that of in CMV group and CMV + PLV group; between-group comparison had significant difference (P<0.01). HFOV + PLV group showed lowest apoptosis indexes. Conclusion: HFOV combined with PLV can suppress lung tissue apoptosis induced by steam inhalation. The Author(s), 2015 High-frequency oscillatory ventilation nationallicence Partial liquid ventilation nationallicence Cell apoptosis nationallicence Inhalation injury nationallicence Zuo Huimin Department of Respiration, The Second Affiliated Hospital of Nanchang University, 330006, Nanchang, China aut Zeng Linxiang Department of Respiration, The Second Affiliated Hospital of Nanchang University, 330006, Nanchang, China aut Guo Guanghua Department of Burns Surgery, The First Affiliated Hospital of Nanchang University, 330006, Nanchang, China aut Zeng Huihong Institute for Basic Medical Sciences, Nanchang University, 330006, Nanchang, China aut Wiener klinische Wochenschrift Springer Vienna 127/15-16(2015-08-01), 606-611 0043-5325 127:15-16<606 2015 127 508 https://doi.org/10.1007/s00508-015-0727-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00508-015-0727-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zuo Huimin Department of Respiration, The Second Affiliated Hospital of Nanchang University, 330006, Nanchang, China aut NATIONALLICENCE 700 1- Zeng Linxiang Department of Respiration, The Second Affiliated Hospital of Nanchang University, 330006, Nanchang, China aut NATIONALLICENCE 700 1- Guo Guanghua Department of Burns Surgery, The First Affiliated Hospital of Nanchang University, 330006, Nanchang, China aut NATIONALLICENCE 700 1- Zeng Huihong Institute for Basic Medical Sciences, Nanchang University, 330006, Nanchang, China aut NATIONALLICENCE 773 0- Wiener klinische Wochenschrift Springer Vienna 127/15-16(2015-08-01), 606-611 0043-5325 127:15-16<606 2015 127 508