caa a22 4500 606241019 CHVBK 20210128101311.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s00508-014-0682-6 doi (NATIONALLICENCE)springer-10.1007/s00508-014-0682-6 Effect of airborne hydrogen peroxide on spores of Clostridium difficile [Elektronische Daten] [Georg Steindl, Anita Fiedler, Steliana Huhulescu, Günther Wewalka, Franz Allerberger] Effekt von gasförmigem Wasserstoffperoxid auf Sporen von Clostridium difficile Summary: Background: Contamination of surfaces by spores of Clostridium difficile is a major factor influencing the spread of healthcare-associated C. difficile infection. The aim of this study was to test the effect of an automated room disinfection system that provides an aerosol of 7.5 % hydrogen peroxide (H2O2) disinfectant, on spores of two different strains of C. difficile, and to evaluate the impact of biological soiling on the efficacy of H2O2 disinfection. Material and method: The strains used were a C. difficile PCR ribotype 027 and a C. difficile ATCC 9689. Spore suspensions of each strain were applied to ceramic tiles and exposed to aerosolized H2O2 at different locations in a test room. Biological soiling was simulated by bovine serum albumin and sheep erythrocytes. At set time points spores were recovered, plated onto Columbia 5 % sheep blood agar, and surviving bacteria were counted as colony-forming units (cfu). Results: No viable spores of either strain were recovered after a 3h exposure to gaseous H2O2. Spores located inside a drawer showed recovery of approximately 1E5cfu/ml for C. difficile ribotype 027 after 1h. In the presence of organic matter, a more than fivefold log reduction compared with not exposed controls could be observed for spores of either strain tested. Conclusion: Appropriate decontamination of surfaces exposed to spores of C. difficile is challenging for conventional cleaning methods. Aerosolized H2O2 delivered by automated room disinfection systems could possibly improve surface decontamination and thereby reduce transmission of healthcare-associated C. difficile infection. Also in the presence of organic matter H2O2 disinfection appears to be an effective adjunct for decontamination of environmental surfaces. Springer-Verlag Wien, 2014 C. difficile nationallicence Spores nationallicence Airborne hydrogen peroxide nationallicence Surface nationallicence Decontamination nationallicence Sporen nationallicence Wasserstoffperoxid nationallicence Oberflächen nationallicence Dekontamination nationallicence Steindl Georg Institute of Medical Microbiology and Hygiene, Austrian Agency for Health and Food Safety, Beethovenstraße 6, 8010, Graz, Austria aut Fiedler Anita Institute of Medical Microbiology and Hygiene, Austrian Agency for Health and Food Safety, Beethovenstraße 6, 8010, Graz, Austria aut Huhulescu Steliana Institute of Medical Microbiology and Hygiene, Austrian Agency for Health and Food Safety, Beethovenstraße 6, 8010, Graz, Austria aut Wewalka Günther Institute of Medical Microbiology and Hygiene, Austrian Agency for Health and Food Safety, Beethovenstraße 6, 8010, Graz, Austria aut Allerberger Franz Institute of Medical Microbiology and Hygiene, Austrian Agency for Health and Food Safety, Beethovenstraße 6, 8010, Graz, Austria aut Wiener klinische Wochenschrift Springer Vienna 127/11-12(2015-06-01), 421-426 0043-5325 127:11-12<421 2015 127 508 https://doi.org/10.1007/s00508-014-0682-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00508-014-0682-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Steindl Georg Institute of Medical Microbiology and Hygiene, Austrian Agency for Health and Food Safety, Beethovenstraße 6, 8010, Graz, Austria aut NATIONALLICENCE 700 1- Fiedler Anita Institute of Medical Microbiology and Hygiene, Austrian Agency for Health and Food Safety, Beethovenstraße 6, 8010, Graz, Austria aut NATIONALLICENCE 700 1- Huhulescu Steliana Institute of Medical Microbiology and Hygiene, Austrian Agency for Health and Food Safety, Beethovenstraße 6, 8010, Graz, Austria aut NATIONALLICENCE 700 1- Wewalka Günther Institute of Medical Microbiology and Hygiene, Austrian Agency for Health and Food Safety, Beethovenstraße 6, 8010, Graz, Austria aut NATIONALLICENCE 700 1- Allerberger Franz Institute of Medical Microbiology and Hygiene, Austrian Agency for Health and Food Safety, Beethovenstraße 6, 8010, Graz, Austria aut NATIONALLICENCE 773 0- Wiener klinische Wochenschrift Springer Vienna 127/11-12(2015-06-01), 421-426 0043-5325 127:11-12<421 2015 127 508