caa a22 4500 606241183 CHVBK 20210128101311.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s00508-015-0721-y doi (NATIONALLICENCE)springer-10.1007/s00508-015-0721-y Serotypes and genotypes of invasive pneumococci in the central part of Slovenia [Elektronische Daten] [Manica Müller Premru, Bojana Beović, Marko Pokorn, Vesna Cvitković Špik] Summary: To investigate epidemiology of invasive pneumococcal disease (IPD) in the central part of Slovenia in a population with no routine pneumococcal vaccination, we carried out serotyping of isolates by sequential multiplex polymerase chain reaction (PCR) and genotyping by repetitive sequence-based PCR (rep-PCR) and some by multilocus sequence typing. IPD was confirmed in 134 (26.5 %) of 510 acutely ill patients, either by a positive blood culture or real-time PCR (rt-PCR). In 94 patients, isolates were available for typing (24 from blood and 70 from nasopharynx). They belonged to 12 different serotypes; the most prevalent were 14 (27.6 % isolates), 9V, 3 (12.7 % each), 7F (9.5 %), 19A, and 1 (7.4 % each) followed by 4, 6A/B, 19F, 23F, 18C, and 33F. Genotyping yielded 34 rep-PCR genotypes and 13 subtypes; six were found in serotype 14, one in 9V, four each in 3, 19A, and 6A/B, three each in 7F and 1, and two each in 4, 19F, 23F, and 18C. Serotype 9V was the most homogenous and 14 and 19A were heterogenous and had two divergent clonal groups each. The most common genotypes belonged to virulent widespread clones, like ST162, ST9, ST15, ST156, ST191, and ST1377; however, sporadic clones were also observed. Springer-Verlag Wien, 2015 Streptococcus pneumoniae nationallicence Invasive disease nationallicence Serotypes nationallicence Genotypes nationallicence Müller Premru Manica Institute of Microbiology and Immunology, Faculty of Medicine, University of Ljubljana, Zaloška 4, 1000, Ljubljana, Slovenia aut Beović Bojana Department of Infectious Diseases, University Medical Centre, Ljubljana, Slovenia aut Pokorn Marko Department of Infectious Diseases, University Medical Centre, Ljubljana, Slovenia aut Cvitković Špik Vesna Institute of Microbiology and Immunology, Faculty of Medicine, University of Ljubljana, Zaloška 4, 1000, Ljubljana, Slovenia aut Wiener klinische Wochenschrift Springer Vienna 127/17-18(2015-09-01), 691-695 0043-5325 127:17-18<691 2015 127 508 https://doi.org/10.1007/s00508-015-0721-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00508-015-0721-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Müller Premru Manica Institute of Microbiology and Immunology, Faculty of Medicine, University of Ljubljana, Zaloška 4, 1000, Ljubljana, Slovenia aut NATIONALLICENCE 700 1- Beović Bojana Department of Infectious Diseases, University Medical Centre, Ljubljana, Slovenia aut NATIONALLICENCE 700 1- Pokorn Marko Department of Infectious Diseases, University Medical Centre, Ljubljana, Slovenia aut NATIONALLICENCE 700 1- Cvitković Špik Vesna Institute of Microbiology and Immunology, Faculty of Medicine, University of Ljubljana, Zaloška 4, 1000, Ljubljana, Slovenia aut NATIONALLICENCE 773 0- Wiener klinische Wochenschrift Springer Vienna 127/17-18(2015-09-01), 691-695 0043-5325 127:17-18<691 2015 127 508