caa a22 4500 606242414 CHVBK 20210128101318.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s12602-014-9171-5 doi (NATIONALLICENCE)springer-10.1007/s12602-014-9171-5 Nisin Incorporated With 2,3-Dihydroxybenzoic Acid in Nanofibers Inhibits Biofilm Formation by a Methicillin-Resistant Strain of Staphylococcus aureus [Elektronische Daten] [Jayesh Ahire, Leon Dicks] The aim of the present study was to determine the effect of nisin, 2,3-dihydroxybenzoic acid (DHBA) and a combination of nisin and DHBA incorporated into nanofibers prepared from poly(d,l-lactide) (PDLLA) and poly(ethylene oxide) (PEO) on biofilm formation of a methicillin-resistant strain of Staphylococcus aureus (strain Xen 31). Biofilm formation decreased by 88% after 24h of exposure to nanofibers containing nisin and DHBA (NDF), compared to a 63% decrease when exposed to nanofibers containing only DHBA (DF) and a 3% decrease when exposed to nanofibers containing only nisin (NF). Planktonic cell numbers of biofilms exposed to nanofibers without nisin or DHBA (CF) and NF increased from no detectable OD595nm readings to 0.35 and 0.3, respectively, within the first 8h of exposure, followed by a steady decline over the following 16h. Planktonic cells of biofilms treated with DF increased from no detectable OD595nm readings to 0.05 after 8h of exposure and remained more-or-less constant for the duration of the experiment. Planktonic cells of biofilms exposed to NDF increased from OD595nm 0.03 after 8h of exposure and to 0.2 over the following 16h. Biofilm formation increased with increasing concentrations of FeCl3·6H2O, which suggests that iron is required for S. aureus Xen 31 to form a biofilm. However, when exposed to NDF, biofilm formation decreased significantly in the presence of increasing concentrations of iron. This suggests that NDF may be used to prevent biofilm formation of MRSA and control infection. Springer Science+Business Media New York, 2014 Biofilms nationallicence MRSA nationallicence Nanofibers nationallicence Iron chelator nationallicence Nisin nationallicence Ahire Jayesh Department of Microbiology, University of Stellenbosch, Private Bag X1, 7602, Matieland, Stellenbosch, South Africa aut Dicks Leon Department of Microbiology, University of Stellenbosch, Private Bag X1, 7602, Matieland, Stellenbosch, South Africa aut Probiotics and Antimicrobial Proteins Springer US; http://www.springer-ny.com 7/1(2015-03-01), 52-59 1867-1306 7:1<52 2015 7 12602 https://doi.org/10.1007/s12602-014-9171-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s12602-014-9171-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ahire Jayesh Department of Microbiology, University of Stellenbosch, Private Bag X1, 7602, Matieland, Stellenbosch, South Africa aut NATIONALLICENCE 700 1- Dicks Leon Department of Microbiology, University of Stellenbosch, Private Bag X1, 7602, Matieland, Stellenbosch, South Africa aut NATIONALLICENCE 773 0- Probiotics and Antimicrobial Proteins Springer US; http://www.springer-ny.com 7/1(2015-03-01), 52-59 1867-1306 7:1<52 2015 7 12602