caa a22 4500 606242430 CHVBK 20210128101318.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s12602-014-9179-x doi (NATIONALLICENCE)springer-10.1007/s12602-014-9179-x Actinobacterial Flora in Feces of Healthy Cottontail Rabbits ( Sylvilagus auduboni ) [Elektronische Daten] [Yu Zhang, Hongming Tan, Qingli Deng, Lixiang Cao] Most known antibiotics from bacteria are produced by Actinobacteria. However, little is known about the community structure and diversity of fecal actinobacteria from rabbit feces. To investigate the actinobacterial community structure in rabbit feces, different actinobacterial-specific primer sets were used to amplify the overlap regions of 16S rRNA genes from the same DNA. At the genus level, 12 actinobacterial genera were detected by the L and S libraries. Arthrobacter, Brachybacterium, Dietzia, Leucobacter, Microbacterium, Promicromonospora and Rhodococcus were detected by L and S libraries. The Nocardioides, Streptomyces and Williamsia were only detected by L library; the Oerskovia and Brevibacterium were only detected by S library. The results indicated that rabbit feces contain diverse nonpathogenic actinobacterial taxa and PCR primer sets could underestimate the actinobacterial diversity besides the DNA extract efficiency. Springer Science+Business Media New York, 2014 Actinobacteria nationallicence Primer set nationallicence Rabbit nationallicence Symbiosis nationallicence Zhang Yu School of Life Sciences, Sun Yat-sen University, 510275, Guangzhou, People's Republic of China aut Tan Hongming School of Life Sciences, Sun Yat-sen University, 510275, Guangzhou, People's Republic of China aut Deng Qingli School of Life Sciences, Sun Yat-sen University, 510275, Guangzhou, People's Republic of China aut Cao Lixiang School of Life Sciences, Sun Yat-sen University, 510275, Guangzhou, People's Republic of China aut Probiotics and Antimicrobial Proteins Springer US; http://www.springer-ny.com 7/1(2015-03-01), 9-13 1867-1306 7:1<9 2015 7 12602 https://doi.org/10.1007/s12602-014-9179-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s12602-014-9179-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Yu School of Life Sciences, Sun Yat-sen University, 510275, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Tan Hongming School of Life Sciences, Sun Yat-sen University, 510275, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Deng Qingli School of Life Sciences, Sun Yat-sen University, 510275, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Cao Lixiang School of Life Sciences, Sun Yat-sen University, 510275, Guangzhou, People's Republic of China aut NATIONALLICENCE 773 0- Probiotics and Antimicrobial Proteins Springer US; http://www.springer-ny.com 7/1(2015-03-01), 9-13 1867-1306 7:1<9 2015 7 12602