caa a22 4500 606242511 CHVBK 20210128101319.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s12602-015-9194-6 doi (NATIONALLICENCE)springer-10.1007/s12602-015-9194-6 Effective Treatment of Staphylococcal Scalded Skin Syndrome with Platelet Microbicidal Protein in CBRB-Rb(8.17)1Iem Mice Model [Elektronische Daten] [Sergey Miroshnikov, Viktor Gritsenko, Iuri Ivanov] Skin and soft-tissue infections are among the most common infections. Staphylococcus aureus may cause a number of toxin-mediated diseases, including staphylococcal scalded skin syndrome (SSSS). The therapeutic efficacy of some antimicrobial peptides was recently evaluated in a mouse model of SSSS. This study is the first in vivo demonstration of the use of PMP to improve outcome of SSSS. Twenty-four CBRB-Rb(8.17)1Iem female mice naturally infected by endogenous S. aureus with SSSS symptoms were used in this work and divided into two equal groups. From neck of each mouse was isolated and identified endogenous exfoliative producing strain of S. aureus. PMP was obtained from human platelets and tested against Bacillus subtilis ATCC 6633. PMP had bactericidal activity against B. subtilis ATTC 6633 and endogenous strain of S. aureus at 2.0±0.5 and 14.5±0.5µg/ml, respectively. At 4weeks, the mice of experimental group were treated subcutaneous near exfoliative zone with 0.2ml of PMP in final concentration 10µg/ml every day. Control mice was injected with 0.2ml 0.9% NaCl. At 1day of experiment maximal zone of alopecia was at PMP-treating group (380±20mm2) in comparison with control group (167±10mm2, p<0.01). At 50day of observation (22nd day after the end of treatment), the square of alopecia in control group was 1220±40mm2 in comparison with 870±17mm2 in experimental group (p<0.01). The antistaphylococcal in vivo activity of PMP demonstrated in present study makes these molecules potentially useful for treatment of SSSS. Springer Science+Business Media New York, 2015 Staphylococcal scalded skin syndrome nationallicence Mice model nationallicence Platelet microbicidal protein nationallicence Treatment nationallicence Miroshnikov Sergey All-Russian Institute of Meat Cattle Breeding, Russian Academy of Agricultural Sciences, Orenburg, Russia aut Gritsenko Viktor Institute of Cellular and Intracellular Symbiosis, Russian Academy of Sciences, Pionerskaya St., 11, 460000, Orenburg, Russia aut Ivanov Iuri Institute of Cellular and Intracellular Symbiosis, Russian Academy of Sciences, Pionerskaya St., 11, 460000, Orenburg, Russia aut Probiotics and Antimicrobial Proteins Springer US; http://www.springer-ny.com 7/3(2015-09-01), 203-206 1867-1306 7:3<203 2015 7 12602 https://doi.org/10.1007/s12602-015-9194-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 brief-communication jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s12602-015-9194-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Miroshnikov Sergey All-Russian Institute of Meat Cattle Breeding, Russian Academy of Agricultural Sciences, Orenburg, Russia aut NATIONALLICENCE 700 1- Gritsenko Viktor Institute of Cellular and Intracellular Symbiosis, Russian Academy of Sciences, Pionerskaya St., 11, 460000, Orenburg, Russia aut NATIONALLICENCE 700 1- Ivanov Iuri Institute of Cellular and Intracellular Symbiosis, Russian Academy of Sciences, Pionerskaya St., 11, 460000, Orenburg, Russia aut NATIONALLICENCE 773 0- Probiotics and Antimicrobial Proteins Springer US; http://www.springer-ny.com 7/3(2015-09-01), 203-206 1867-1306 7:3<203 2015 7 12602