caa a22 4500 606242538 CHVBK 20210128101319.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s12602-015-9197-3 doi (NATIONALLICENCE)springer-10.1007/s12602-015-9197-3 Molecular Characterization and In Silico Analysis of Defensin From Tor putitora (Hamilton) [Elektronische Daten] [Preeti Chaturvedi, Meenakshi Dhanik, Amit Pande] Antimicrobial peptides (AMPs) are vital constituents of innate immune system. In fish, one of the most important AMP is β-defensin that has a potential to activate the adaptive immune system. β-defensin is a cysteine-arginine-rich cationic AMP with broad spectrum activity against microbes. In this study, we identified cloned and characterized β-defensin 1 from Tor putitora, a cold water fish species also known as mahseer. An open reading frame of β-defensin 1 was amplified, cloned and sequenced which encodes a peptide of 67 amino acid residues. The pro-peptide includes a signal peptide comprising 24 amino acids as predicted by Signal P along with a mature peptide of 43 amino acid residues. Tor putitora β-defensin 1 (TP-βdf1) has a molecular weight of 4.6kDa with a pI of 8.35. Six cysteine residues are present in the mature peptide which is a characteristic feature of defensins. All six cysteine residues are involved in the formation of three intra-molecular disulfide bonds. Three-dimensional modeling of mature peptide of TP-βdf1 was carried out using Modeller 9.10, and validated TP-βdf1 model revealed three β-sheets. The cysteine residues form three disulfide bonds in the pattern of Cys1-Cys5, Cys2-Cys4, Cys3-Cys6 stabilizing the β-sheet. Structural analysis revealed three β-strands and an α-helix at the N terminus. Phylogenetic analysis revealed that the β-defensin 1 of Tor putitora was close to Megalobrama amblycephala which possibly suggests that TP-βdf1 peptide sequence is quite similar to β-defensin peptide sequences of carps and minnows. Springer Science+Business Media New York, 2015 Antimicrobial peptides (AMPs) nationallicence β-Defensin nationallicence Fish innate immunity nationallicence Tor putitora β-defensin 1 (TP-βdf1) nationallicence Immuno-modulators nationallicence Chaturvedi Preeti Directorate of Coldwater Fisheries Research, 263136, Bhimtal Nainital, Uttarakhand, India aut Dhanik Meenakshi Directorate of Coldwater Fisheries Research, 263136, Bhimtal Nainital, Uttarakhand, India aut Pande Amit Directorate of Coldwater Fisheries Research, 263136, Bhimtal Nainital, Uttarakhand, India aut Probiotics and Antimicrobial Proteins Springer US; http://www.springer-ny.com 7/3(2015-09-01), 207-215 1867-1306 7:3<207 2015 7 12602 https://doi.org/10.1007/s12602-015-9197-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s12602-015-9197-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chaturvedi Preeti Directorate of Coldwater Fisheries Research, 263136, Bhimtal Nainital, Uttarakhand, India aut NATIONALLICENCE 700 1- Dhanik Meenakshi Directorate of Coldwater Fisheries Research, 263136, Bhimtal Nainital, Uttarakhand, India aut NATIONALLICENCE 700 1- Pande Amit Directorate of Coldwater Fisheries Research, 263136, Bhimtal Nainital, Uttarakhand, India aut NATIONALLICENCE 773 0- Probiotics and Antimicrobial Proteins Springer US; http://www.springer-ny.com 7/3(2015-09-01), 207-215 1867-1306 7:3<207 2015 7 12602