caa a22 4500 606242546 CHVBK 20210128101319.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s12602-015-9193-7 doi (NATIONALLICENCE)springer-10.1007/s12602-015-9193-7 Scaling Up the Production of Recombinant Antimicrobial Plantaricin E from a Heterologous Host, Escherichia coli [Elektronische Daten] [Gargi Pal, Sheela Srivastava] Enhanced production of heterologously expressed plantaricin (plnE) from Escherichia coli BL21 (DE3) was achieved from a small- to large-scale batch culture. Starting from a 15-ml shake-flask culture grown in Luria-Bertani (LB) broth, the protein expression could be scaled up using 50ml, 100ml, 1l, and 2l batch culture. Using similar condition, plantaricin E (PlnE) was successfully expressed in a 30-l stirred fermenter. The protein was expressed as TRX-(His)6-fusion protein and separated by Ni2+ affinity chromatography. Growth in two complex media, LB and Terrific broth (TB), was optimized and compared for the production of PlnE, which was higher in LB in comparison with that of TB. In the fermenter, 140 and 180mg of PlnE could be produced from 12l of culture volume at 30 and 25°C, respectively. The yield of heterologously purified PlnE was found to be 1.2-1.5%, which was much higher in comparison with the plantaricins produced from the native strain of Lactobacillus plantarum (0.3-0.7%). Overproduction of PlnE with the help of heterologous expression can overcome the constraint of the low yield from producer strain and provides an easy and low-cost strategy for large-scale production. Springer Science+Business Media New York, 2015 Batch cultivation nationallicence Growth media nationallicence Plantaricin nationallicence Fermenter nationallicence Pal Gargi Department of Genetics, University of Delhi, South Campus, 110021, New Delhi, India aut Srivastava Sheela Department of Genetics, University of Delhi, South Campus, 110021, New Delhi, India aut Probiotics and Antimicrobial Proteins Springer US; http://www.springer-ny.com 7/3(2015-09-01), 216-221 1867-1306 7:3<216 2015 7 12602 https://doi.org/10.1007/s12602-015-9193-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s12602-015-9193-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pal Gargi Department of Genetics, University of Delhi, South Campus, 110021, New Delhi, India aut NATIONALLICENCE 700 1- Srivastava Sheela Department of Genetics, University of Delhi, South Campus, 110021, New Delhi, India aut NATIONALLICENCE 773 0- Probiotics and Antimicrobial Proteins Springer US; http://www.springer-ny.com 7/3(2015-09-01), 216-221 1867-1306 7:3<216 2015 7 12602