caa a22 4500 606242589 CHVBK 20210128101319.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s12602-015-9198-2 doi (NATIONALLICENCE)springer-10.1007/s12602-015-9198-2 Molecular Characterization and Phylogenetic Analysis of Novel Isoform of Anti-lipopolysaccharide Factor from the Mantis Shrimp, Miyakea nepa [Elektronische Daten] [K. Sruthy, E. Chaithanya, Naveen Sathyan, Aishwarya Nair, Swapna Antony, I. Bright Singh, Rosamma Philip] Anti-lipopolysaccharide factor (ALF) is a cationic anti-microbial peptide representing humoral defence system exhibiting a diverse spectrum of activity against microbial pathogens, including gram-negative and gram-positive bacteria, fungi, parasites and viruses. In this study, we identified and characterized a novel ALF homologue (MnALF) encoding cDNA sequence from the haemocytes of stomatopod mantis shrimp Miyakea nepa. The deduced peptide of MnALF encoded for a 123-amino acid peptide with a 25-residue signal peptide containing selenocysteine followed by a highly cationic mature peptide comprised of a putative LPS-binding domain flanked by two cysteine residues. BLAST analysis of MnALF showed that it exhibits identity to crustacean and limulid ALFs. The mature peptide of MnALF has a net charge of +7 and predicted molecular weight of 10.998kDa with a theoretical isoelectric point (pI) of 9.93. Spatial structure of MnALF comprises three α-helices packed against a four-stranded β-sheet of which two were linked by a disulphide bond to form an amphipathic loop similar to the structure of Penaeus monodon, ALF-Pm3. All these features suggest that MnALF could play an imperative role in the innate defence mechanism of M. nepa. To our knowledge, this study accounts for the first report of an anti-microbial peptide from the order stomatopoda. Springer Science+Business Media New York, 2015 Anti-microbial peptides nationallicence Anti-lipopolysaccharide factor nationallicence Stomatopod crustacean nationallicence Miyakea nepa nationallicence Selenocysteine nationallicence SPase : Signal peptidase nationallicence SPPase : Signal peptide peptidase nationallicence Sruthy K. Department of Marine Biology, Microbiology and Biochemistry, School of Marine Sciences, Cochin University of Science and Technology, Fine Arts Avenue, 682 016, Kochi, Kerala, India aut Chaithanya E. Department of Marine Biology, Microbiology and Biochemistry, School of Marine Sciences, Cochin University of Science and Technology, Fine Arts Avenue, 682 016, Kochi, Kerala, India aut Sathyan Naveen Department of Marine Biology, Microbiology and Biochemistry, School of Marine Sciences, Cochin University of Science and Technology, Fine Arts Avenue, 682 016, Kochi, Kerala, India aut Nair Aishwarya Department of Marine Biology, Microbiology and Biochemistry, School of Marine Sciences, Cochin University of Science and Technology, Fine Arts Avenue, 682 016, Kochi, Kerala, India aut Antony Swapna National Centre for Aquatic Animal Health, Cochin University of Science and Technology, 682 016, Kochi, Kerala, India aut Bright Singh I. National Centre for Aquatic Animal Health, Cochin University of Science and Technology, 682 016, Kochi, Kerala, India aut Philip Rosamma Department of Marine Biology, Microbiology and Biochemistry, School of Marine Sciences, Cochin University of Science and Technology, Fine Arts Avenue, 682 016, Kochi, Kerala, India aut Probiotics and Antimicrobial Proteins Springer US; http://www.springer-ny.com 7/4(2015-12-01), 275-283 1867-1306 7:4<275 2015 7 12602 https://doi.org/10.1007/s12602-015-9198-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s12602-015-9198-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sruthy K. Department of Marine Biology, Microbiology and Biochemistry, School of Marine Sciences, Cochin University of Science and Technology, Fine Arts Avenue, 682 016, Kochi, Kerala, India aut NATIONALLICENCE 700 1- Chaithanya E. Department of Marine Biology, Microbiology and Biochemistry, School of Marine Sciences, Cochin University of Science and Technology, Fine Arts Avenue, 682 016, Kochi, Kerala, India aut NATIONALLICENCE 700 1- Sathyan Naveen Department of Marine Biology, Microbiology and Biochemistry, School of Marine Sciences, Cochin University of Science and Technology, Fine Arts Avenue, 682 016, Kochi, Kerala, India aut NATIONALLICENCE 700 1- Nair Aishwarya Department of Marine Biology, Microbiology and Biochemistry, School of Marine Sciences, Cochin University of Science and Technology, Fine Arts Avenue, 682 016, Kochi, Kerala, India aut NATIONALLICENCE 700 1- Antony Swapna National Centre for Aquatic Animal Health, Cochin University of Science and Technology, 682 016, Kochi, Kerala, India aut NATIONALLICENCE 700 1- Bright Singh I. National Centre for Aquatic Animal Health, Cochin University of Science and Technology, 682 016, Kochi, Kerala, India aut NATIONALLICENCE 700 1- Philip Rosamma Department of Marine Biology, Microbiology and Biochemistry, School of Marine Sciences, Cochin University of Science and Technology, Fine Arts Avenue, 682 016, Kochi, Kerala, India aut NATIONALLICENCE 773 0- Probiotics and Antimicrobial Proteins Springer US; http://www.springer-ny.com 7/4(2015-12-01), 275-283 1867-1306 7:4<275 2015 7 12602