caa a22 4500 606242597 CHVBK 20210128101319.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s12602-015-9202-x doi (NATIONALLICENCE)springer-10.1007/s12602-015-9202-x Isolation, Identification and Partial Characterization of a Lactobacillus casei Strain with Bile Salt Hydrolase Activity from Pulque [Elektronische Daten] [R. González-Vázquez, A. Azaola-Espinosa, L. Mayorga-Reyes, L. Reyes-Nava, N. Shah, Y. Rivera-Espinoza] The aim of this study was to isolate, from pulque, Lactobacillus spp. capable of survival in simulated gastrointestinal stress conditions. Nine Gram-positive rods were isolated; however, only one strain (J57) shared identity with Lactobacillus and was registered as Lactobacillus casei J57 (GenBank accession: JN182264). The other strains were identified as Bacillus spp. The most significant observation during the test of tolerance to simulated gastrointestinal conditions (acidity, gastric juice and bile salts) was that L. casei J57 showed a rapid decrease (p≤0.05) in the viable population at 0h. Bile salts were the stress condition that most affected its survival, from which deoxycholic acid and the mix of bile salts (oxgall) were the most toxic. L. casei J57 showed bile salt hydrolase activity over primary and secondary bile salts as follows: 44.91, 671.72, 45.27 and 61.57U/mg to glycocholate, taurocholate, glycodeoxycholate and taurodeoxycholate. In contrast, the control strain (L. casei Shirota) only showed activity over tauroconjugates. These results suggest that L. casei J57 shows potential for probiotic applications. Springer Science+Business Media New York, 2015 Pulque nationallicence Lactobacillus nationallicence Bile salt hydrolase nationallicence Probiotics nationallicence González-Vázquez R. Departamento de Sistemas Biológicos, Maestría en Ciencias Farmacéuticas, Universidad Autónoma Metropolitana, Calzada del Hueso, 1100, Coyoacan, 04960, Mexico City, Mexico aut Azaola-Espinosa A. Departamento de Sistemas Biológicos, Maestría en Ciencias Farmacéuticas, Universidad Autónoma Metropolitana, Calzada del Hueso, 1100, Coyoacan, 04960, Mexico City, Mexico aut Mayorga-Reyes L. Departamento de Sistemas Biológicos, Maestría en Ciencias Farmacéuticas, Universidad Autónoma Metropolitana, Calzada del Hueso, 1100, Coyoacan, 04960, Mexico City, Mexico aut Reyes-Nava L. Departamento de Graduados e Investigación en Alimentos, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Carpio y Plan de Ayala, 11340, Mexico City, Mexico aut Shah N. Food and Nutritional Science Division, School of Biological Sciences, The University of Hong Kong, Pokfulam Road, Pok Fu Lam, Hong Kong aut Rivera-Espinoza Y. Departamento de Graduados e Investigación en Alimentos, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Carpio y Plan de Ayala, 11340, Mexico City, Mexico aut Probiotics and Antimicrobial Proteins Springer US; http://www.springer-ny.com 7/4(2015-12-01), 242-248 1867-1306 7:4<242 2015 7 12602 https://doi.org/10.1007/s12602-015-9202-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s12602-015-9202-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- González-Vázquez R. Departamento de Sistemas Biológicos, Maestría en Ciencias Farmacéuticas, Universidad Autónoma Metropolitana, Calzada del Hueso, 1100, Coyoacan, 04960, Mexico City, Mexico aut NATIONALLICENCE 700 1- Azaola-Espinosa A. Departamento de Sistemas Biológicos, Maestría en Ciencias Farmacéuticas, Universidad Autónoma Metropolitana, Calzada del Hueso, 1100, Coyoacan, 04960, Mexico City, Mexico aut NATIONALLICENCE 700 1- Mayorga-Reyes L. Departamento de Sistemas Biológicos, Maestría en Ciencias Farmacéuticas, Universidad Autónoma Metropolitana, Calzada del Hueso, 1100, Coyoacan, 04960, Mexico City, Mexico aut NATIONALLICENCE 700 1- Reyes-Nava L. Departamento de Graduados e Investigación en Alimentos, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Carpio y Plan de Ayala, 11340, Mexico City, Mexico aut NATIONALLICENCE 700 1- Shah N. Food and Nutritional Science Division, School of Biological Sciences, The University of Hong Kong, Pokfulam Road, Pok Fu Lam, Hong Kong aut NATIONALLICENCE 700 1- Rivera-Espinoza Y. Departamento de Graduados e Investigación en Alimentos, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Carpio y Plan de Ayala, 11340, Mexico City, Mexico aut NATIONALLICENCE 773 0- Probiotics and Antimicrobial Proteins Springer US; http://www.springer-ny.com 7/4(2015-12-01), 242-248 1867-1306 7:4<242 2015 7 12602