caa a22 4500 606242635 CHVBK 20210128101319.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s12602-015-9191-9 doi (NATIONALLICENCE)springer-10.1007/s12602-015-9191-9 Inactivated Probiotic Bacteria Stimulate Cellular Immune Responses of Catla, Catla catla (Hamilton) In Vitro [Elektronische Daten] [Dibyendu Kamilya, Arunjyoti Baruah, Timothy Sangma, Supratim Chowdhury, Prasenjit Pal] In the present study, we investigated the in vitro immunostimulatory effects of inactivated form of two potential probiotics, Bacillus amyloliquefaciens FPTB16 and B. subtilis FPTB13 in catla (Catla catla). Catla head kidney leukocytes (HK) were incubated with the bacteria alive or inactivated with heat shock (2h at 60°C), UV light (for 2.5h) and formalin (1.0%, v/v) treatment (for 24h at 4°C) at different concentrations (107, 108 and 109cellsml−1). After incubation, different cellular immune parameters such as respiratory burst activity, nitric oxide production, leukocyte peroxidase content and proliferative response were analyzed. The inactivated probiotic preparations stimulated all the cellular immune parameters of catla HK leukocytes in vitro. Among the different inactivated preparations, heat-treated form exhibited the best result. The lowest dose (107cellsml−1) of both the strains showed the maximum stimulation. The results collectively suggest the efficacy of inactivated preparations to be used as immunostimulant in aquaculture. Springer Science+Business Media New York, 2015 Probiotics nationallicence Bacillus amyloliquefaciens FPTB16 nationallicence Bacillus subtilis FPTB13 nationallicence Catla ( Catla catla ) nationallicence In vitro immunomodulation nationallicence Kamilya Dibyendu Department of Fish Health and Environment, College of Fisheries, Central Agricultural University, 799 210, Lembucherra, Tripura, India aut Baruah Arunjyoti Department of Fish Health and Environment, College of Fisheries, Central Agricultural University, 799 210, Lembucherra, Tripura, India aut Sangma Timothy Department of Fish Health and Environment, College of Fisheries, Central Agricultural University, 799 210, Lembucherra, Tripura, India aut Chowdhury Supratim Department of Fish Processing Technology, Faculty of Fishery Sciences, West Bengal University of Animal and Fishery Sciences, 5-Budherhat Road, Chakgaria, P. O. Panchasayar, 700 094, Kolkata, West Bengal, India aut Pal Prasenjit Department of Fisheries Extension, College of Fisheries, Central Agricultural University, 799 210, Lembucherra, Tripura, India aut Probiotics and Antimicrobial Proteins Springer US; http://www.springer-ny.com 7/2(2015-06-01), 101-106 1867-1306 7:2<101 2015 7 12602 https://doi.org/10.1007/s12602-015-9191-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s12602-015-9191-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kamilya Dibyendu Department of Fish Health and Environment, College of Fisheries, Central Agricultural University, 799 210, Lembucherra, Tripura, India aut NATIONALLICENCE 700 1- Baruah Arunjyoti Department of Fish Health and Environment, College of Fisheries, Central Agricultural University, 799 210, Lembucherra, Tripura, India aut NATIONALLICENCE 700 1- Sangma Timothy Department of Fish Health and Environment, College of Fisheries, Central Agricultural University, 799 210, Lembucherra, Tripura, India aut NATIONALLICENCE 700 1- Chowdhury Supratim Department of Fish Processing Technology, Faculty of Fishery Sciences, West Bengal University of Animal and Fishery Sciences, 5-Budherhat Road, Chakgaria, P. O. Panchasayar, 700 094, Kolkata, West Bengal, India aut NATIONALLICENCE 700 1- Pal Prasenjit Department of Fisheries Extension, College of Fisheries, Central Agricultural University, 799 210, Lembucherra, Tripura, India aut NATIONALLICENCE 773 0- Probiotics and Antimicrobial Proteins Springer US; http://www.springer-ny.com 7/2(2015-06-01), 101-106 1867-1306 7:2<101 2015 7 12602