caa a22 4500 606242651 CHVBK 20210128101319.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s12602-015-9190-x doi (NATIONALLICENCE)springer-10.1007/s12602-015-9190-x Characterization of Deep Sea Fish Gut Bacteria with Antagonistic Potential, from Centroscyllium fabricii (Deep Sea Shark) [Elektronische Daten] [E. Bindiya, K. Tina, Subin Raghul, Sarita Bhat] The bacterial isolates from Centroscyllium fabricii (deep sea shark) gut were screened for antagonistic activity by cross-streak method and disc diffusion assay. This study focuses on strain BTSS-3, which showed antimicrobial activity against pathogenic bacteria including Salmonella Typhimurium, Proteus vulgaris, Clostridium perfringens, Staphylococcus aureus, Bacillus cereus, Bacillus circulans, Bacillus macerans and Bacillus pumilus. BTSS3 was subjected to phenotypic characterization using biochemical tests, SEM imaging, exoenzyme profiling and antibiotic susceptibility tests. Comparative 16S rDNA gene sequence analysis indicated that this strain belonged to the genus Bacillus, with high (98%) similarity to 16S rDNA sequences of Bacillus amyloliquefaciens. The chemical nature of the antibacterial substance was identified by treatment with proteolytic enzymes. The antibacterial activity was reduced by the action of these enzymes pointing out its peptide nature. It was observed from the growth and production kinetics that the bacteriocin was produced in the eighth hour of incubation, i.e., during the mid-log growth phase of the bacteria. Springer Science+Business Media New York, 2015 Bacillus amyloliquefaciens nationallicence Deep sea shark nationallicence Bacteriocin nationallicence Phenotypic identification nationallicence Genotypic identification nationallicence Bindiya E. Department of Biotechnology, Cochin University of Science and Technology, 682 022, Cochin, India aut Tina K. Department of Biotechnology, Cochin University of Science and Technology, 682 022, Cochin, India aut Raghul Subin Department of Biotechnology, Cochin University of Science and Technology, 682 022, Cochin, India aut Bhat Sarita Department of Biotechnology, Cochin University of Science and Technology, 682 022, Cochin, India aut Probiotics and Antimicrobial Proteins Springer US; http://www.springer-ny.com 7/2(2015-06-01), 157-163 1867-1306 7:2<157 2015 7 12602 https://doi.org/10.1007/s12602-015-9190-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s12602-015-9190-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bindiya E. Department of Biotechnology, Cochin University of Science and Technology, 682 022, Cochin, India aut NATIONALLICENCE 700 1- Tina K. Department of Biotechnology, Cochin University of Science and Technology, 682 022, Cochin, India aut NATIONALLICENCE 700 1- Raghul Subin Department of Biotechnology, Cochin University of Science and Technology, 682 022, Cochin, India aut NATIONALLICENCE 700 1- Bhat Sarita Department of Biotechnology, Cochin University of Science and Technology, 682 022, Cochin, India aut NATIONALLICENCE 773 0- Probiotics and Antimicrobial Proteins Springer US; http://www.springer-ny.com 7/2(2015-06-01), 157-163 1867-1306 7:2<157 2015 7 12602