caa a22 4500 60624266X CHVBK 20210128101319.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s12602-015-9184-8 doi (NATIONALLICENCE)springer-10.1007/s12602-015-9184-8 In Vitro Evaluation of Probiotic Properties of Lactic Acid Bacteria from the Gut of Labeo rohita and Catla catla [Elektronische Daten] [Tapasa Sahoo, Prasant Jena, Nidhi Nagar, Amiya Patel, Sriram Seshadri] We report the evaluation of probiotic properties of potent lactic acid bacteria (LAB) from the gut of freshwater fishes, Labeo rohita and Catla catla, for eventually developing probiotic strains for the prevention of bacterial infections in aquaculture and food preservation. Five different LAB strains were isolated and characterized for their probiotic properties. Based on physiological, morphological and biochemical characteristics, three isolates from Labeo rohita and two from Catla catla were identified as putative probiotics and were denoted as LR11, LR14 and LR16 and CC3 and CC4, respectively. Isolates CC3 and CC4 were acid (pH 2.5) and bile salt (0.3% oxygall) tolerant and exhibited strong antibacterial activities against all pathogens including Aeromonas hydrophila. In addition, all LAB isolates were susceptible to tested antibiotics, except CC3 and CC4 which were vancomycin resistant. Furthermore, the isolates CC3 and CC4 showed significantly higher in vitro cell surface properties, i.e., hydrophobicity, auto- and co-aggregation. Biochemical tests, PCR detection and 16S rRNA sequence analysis established that LR11, LR14, LR16, CC3 and CC4 are Enterococcus avium TSU11, Enterococcus pseudoavium TSU14, Enterococcus raffinosus TSU16, Lactobacillus gasseri TSU3 and Lactobacillus animalis TSU4, respectively. Studies revealed that, Lactobacillus gasseri TSU3 and Lactobacillus animalis TSU4 are ideal probiotic candidates for its use in aquaculture and require further exploratory in vivo evaluation and safety studies. Springer Science+Business Media New York, 2015 Aquaculture nationallicence Probiotics nationallicence Freshwater fish nationallicence Fish gut nationallicence Antimicrobial activity nationallicence A. hydrophila : Aeromonas hydrophila nationallicence B. : Bacillus nationallicence BHI : Brain heart infusion nationallicence C. : Carnobacterium nationallicence CFS : Cell-free supernatants nationallicence CFU : Colony-forming units nationallicence E. : Enterococcus nationallicence EFSA : European Food Safety Authority nationallicence E. coli : Escherichia coli nationallicence FAO : Food and Agriculture Organizations nationallicence GRAS : Generally regarded as safe nationallicence LAB : Lactic acid bacteria nationallicence L. : Lactobacillus nationallicence L. acidophilus : Lactobacillus acidophilus nationallicence LB : Luria-Bertani nationallicence MRS : DeMan Rogosa Sharpe nationallicence MTCC : Microbial Type Culture Collections nationallicence NDCC : National Dairy Culture Collections nationallicence OD : Optical density nationallicence PBS : Phosphate buffered saline nationallicence P. aeruginosa : Pseudomonas aeruginosa nationallicence S. paratyphi : Salmonella paratyphi nationallicence S. Typhimurium : Salmonella Typhimurium nationallicence S. flexneri : Shigella flexneri nationallicence S. aureus : Staphylococcus aureus nationallicence UPEC : Uropathogenic E. coli nationallicence Sahoo Tapasa School of Life Sciences, Sambalpur University, Jyotivihar, Burla, 768019, Sambalpur, Odisha, India aut Jena Prasant Institute of Science, Nirma University, Sarkhej-Gandhinagar Highway, 382481, Ahmedabad, Gujarat, India aut Nagar Nidhi Institute of Science, Nirma University, Sarkhej-Gandhinagar Highway, 382481, Ahmedabad, Gujarat, India aut Patel Amiya School of Life Sciences, Sambalpur University, Jyotivihar, Burla, 768019, Sambalpur, Odisha, India aut Seshadri Sriram Institute of Science, Nirma University, Sarkhej-Gandhinagar Highway, 382481, Ahmedabad, Gujarat, India aut Probiotics and Antimicrobial Proteins Springer US; http://www.springer-ny.com 7/2(2015-06-01), 126-136 1867-1306 7:2<126 2015 7 12602 https://doi.org/10.1007/s12602-015-9184-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s12602-015-9184-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sahoo Tapasa School of Life Sciences, Sambalpur University, Jyotivihar, Burla, 768019, Sambalpur, Odisha, India aut NATIONALLICENCE 700 1- Jena Prasant Institute of Science, Nirma University, Sarkhej-Gandhinagar Highway, 382481, Ahmedabad, Gujarat, India aut NATIONALLICENCE 700 1- Nagar Nidhi Institute of Science, Nirma University, Sarkhej-Gandhinagar Highway, 382481, Ahmedabad, Gujarat, India aut NATIONALLICENCE 700 1- Patel Amiya School of Life Sciences, Sambalpur University, Jyotivihar, Burla, 768019, Sambalpur, Odisha, India aut NATIONALLICENCE 700 1- Seshadri Sriram Institute of Science, Nirma University, Sarkhej-Gandhinagar Highway, 382481, Ahmedabad, Gujarat, India aut NATIONALLICENCE 773 0- Probiotics and Antimicrobial Proteins Springer US; http://www.springer-ny.com 7/2(2015-06-01), 126-136 1867-1306 7:2<126 2015 7 12602