caa a22 4500 606242678 CHVBK 20210128101320.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s12602-015-9189-3 doi (NATIONALLICENCE)springer-10.1007/s12602-015-9189-3 Use of Probiotic Bacillus spp. in Rotifer ( Brachionus plicatilis ) and Artemia ( Artemia urmiana ) Enrichment: Effects on Growth and Survival of Pacific White Shrimp, Litopenaeus vannamei , Larvae [Elektronische Daten] [Hadi Jamali, Ahmad Imani, Daruosh Abdollahi, Reza Roozbehfar, Amin Isari] This study was to evaluate the effect of a preparation of Bacillus probiotic (Bacillus licheniformis and B. subtilis, 1:1) on growth and survival rate of Pacific white shrimp, Litopenaeus vannamei larvae. The larvae were fed on Artemia urmiana nauplii and Brachionus plicatilis enriched with the probiotic preparation at 1×106CFUmL−1 rate. The experimental setup was completely randomized design comprised of six treatments, namely solo Artemia nauplii (A) or rotifer (R), Artemia nauplii and rotifer without any enrichment (A+R), Artemia nauplii enrichment with probiotic bacilli (Bacillus licheniformis and B. subtilis) (A+B), rotifer enrichment with probiotic bacilli (R+B) and enriched Artemia nauplii and rotifer (A+R+B). All treatments were performed in triplicate. Chemical parameters of rearing water viz. pH, salinity and temperature were 7.5-8, 30-31 ppt and 31-32°C, respectively. Photoperiod was 16L:8D. Shrimp larvae were fed Artemia nauplii and rotifers at 5-20 and 10-40 individuals per shrimp larvae four times a day, respectively. Growth and survival rate of larvae were determined at MII, MIII, PL1, PL4, PL7 and PL10 stages. Larvae in A+R+B treatment showed the highest total length (10.89±0.51mm), weight (674±73μg) and survival rate (65%±3.5). Lowest total length, weight and survival rate (7.96±0.63mm, 493±52μg and 24.5±2.4%, respectively) were recorded in treatment B larvae. We concluded that Bacillus probiotic can improve growth and survival rate of Pacific white shrimp larvae without conceivably undesirable effects. Springer Science+Business Media New York, 2015 Bacillus nationallicence Shrimp nationallicence Litopenaeus vannamei nationallicence Artemia urmiana nationallicence Brachionus plicatilis nationallicence Jamali Hadi Department of Fisheries, Urmia University, Urmia, Iran aut Imani Ahmad Department of Fisheries, Urmia University, Urmia, Iran aut Abdollahi Daruosh Department of Fisheries, Gonbad Kavous University, Gonbad Kavous, Iran aut Roozbehfar Reza Department of Fisheries, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran aut Isari Amin Department of Fisheries, Islamic Azad University, Ahvaz Branch, Ahvaz, Iran aut Probiotics and Antimicrobial Proteins Springer US; http://www.springer-ny.com 7/2(2015-06-01), 118-125 1867-1306 7:2<118 2015 7 12602 https://doi.org/10.1007/s12602-015-9189-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s12602-015-9189-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jamali Hadi Department of Fisheries, Urmia University, Urmia, Iran aut NATIONALLICENCE 700 1- Imani Ahmad Department of Fisheries, Urmia University, Urmia, Iran aut NATIONALLICENCE 700 1- Abdollahi Daruosh Department of Fisheries, Gonbad Kavous University, Gonbad Kavous, Iran aut NATIONALLICENCE 700 1- Roozbehfar Reza Department of Fisheries, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran aut NATIONALLICENCE 700 1- Isari Amin Department of Fisheries, Islamic Azad University, Ahvaz Branch, Ahvaz, Iran aut NATIONALLICENCE 773 0- Probiotics and Antimicrobial Proteins Springer US; http://www.springer-ny.com 7/2(2015-06-01), 118-125 1867-1306 7:2<118 2015 7 12602