caa a22 4500 606242694 CHVBK 20210128101320.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s12602-015-9185-7 doi (NATIONALLICENCE)springer-10.1007/s12602-015-9185-7 Optimization of Culture Conditions for Mass Production of the Probiotics Pseudomonas MCCB 102 and 103 Antagonistic to Pathogenic Vibrios in Aquaculture [Elektronische Daten] [R. Preetha, K. Vijayan, N. Jayapraksh, S. Alavandi, T. Santiago, I. Bright Singh] Rapid growth of shrimp farming industry is affected by the recurrence of diverse diseases, among which vibriosis is predominant. Eco-friendly disease management strategy by the application of antagonistic probiotics is widely accepted. In the present study, culture conditions of antagonistic probiotics, Pseudomonas MCCB 102 and 103, were optimized to enhance their biomass production and antagonistic activity against the shrimp pathogen V. harveyi MCCB 111. Primarily, one-dimensional screening was carried out to fix the optimum range of sodium chloride concentration, pH and temperature. The second step optimization was done using a full-factorial central composite design of response surface methodology. As per the model, 12.9g/L sodium chloride and pH 6.5 for Pseudomonas MCCB 102, and 5g/L sodium chloride and pH 7 for Pseudomonas MCCB 103 were found to be ideal to maximize antagonistic activity. However, optimum temperature was the same (25°C) for both isolates. Finally, the models were experimentally validated for enhanced biomass production and antagonistic activity. The optima for biomass and antagonistic activity were more or less the same, suggesting the possible influence of biomass on antagonistic activity. Springer Science+Business Media New York, 2015 Probiotic nationallicence Pseudomonas nationallicence Antagonistic activity nationallicence Vibrios nationallicence Response surface methodology nationallicence Central composite design nationallicence Preetha R. National Centre for Aquatic Animal Health, Cochin University of Science and Technology, Lakeside Campus, Fine Arts Avenue, 682016, Cochin, Kerala, India aut Vijayan K. Central Marine Fisheries Research Institute (Indian Council of Agriculture Research), 682018, Ernakulam North, Cochin, Kerala, India aut Jayapraksh N. National Centre for Aquatic Animal Health, Cochin University of Science and Technology, Lakeside Campus, Fine Arts Avenue, 682016, Cochin, Kerala, India aut Alavandi S. Central Institute of Brackishwater Aquaculture (Indian Council of Agriculture Research), 600028, Chennai, Tamil Nadu, India aut Santiago T. Central Institute of Brackishwater Aquaculture (Indian Council of Agriculture Research), 600028, Chennai, Tamil Nadu, India aut Bright Singh I. National Centre for Aquatic Animal Health, Cochin University of Science and Technology, Lakeside Campus, Fine Arts Avenue, 682016, Cochin, Kerala, India aut Probiotics and Antimicrobial Proteins Springer US; http://www.springer-ny.com 7/2(2015-06-01), 137-145 1867-1306 7:2<137 2015 7 12602 https://doi.org/10.1007/s12602-015-9185-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s12602-015-9185-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Preetha R. National Centre for Aquatic Animal Health, Cochin University of Science and Technology, Lakeside Campus, Fine Arts Avenue, 682016, Cochin, Kerala, India aut NATIONALLICENCE 700 1- Vijayan K. Central Marine Fisheries Research Institute (Indian Council of Agriculture Research), 682018, Ernakulam North, Cochin, Kerala, India aut NATIONALLICENCE 700 1- Jayapraksh N. National Centre for Aquatic Animal Health, Cochin University of Science and Technology, Lakeside Campus, Fine Arts Avenue, 682016, Cochin, Kerala, India aut NATIONALLICENCE 700 1- Alavandi S. Central Institute of Brackishwater Aquaculture (Indian Council of Agriculture Research), 600028, Chennai, Tamil Nadu, India aut NATIONALLICENCE 700 1- Santiago T. Central Institute of Brackishwater Aquaculture (Indian Council of Agriculture Research), 600028, Chennai, Tamil Nadu, India aut NATIONALLICENCE 700 1- Bright Singh I. National Centre for Aquatic Animal Health, Cochin University of Science and Technology, Lakeside Campus, Fine Arts Avenue, 682016, Cochin, Kerala, India aut NATIONALLICENCE 773 0- Probiotics and Antimicrobial Proteins Springer US; http://www.springer-ny.com 7/2(2015-06-01), 137-145 1867-1306 7:2<137 2015 7 12602