caa a22 4500 606242716 CHVBK 20210128101320.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s12602-015-9186-6 doi (NATIONALLICENCE)springer-10.1007/s12602-015-9186-6 Probiotic Potential of Lactobacillus Strains Isolated from Sorghum-Based Traditional Fermented Food [Elektronische Daten] [K. Poornachandra Rao, G. Chennappa, U. Suraj, H. Nagaraja, A. Charith Raj, M. Sreenivasa] Sorghum-based traditional fermented food was screened for potential probiotic lactic acid bacteria. The isolates were identified by biochemical, physiological and genetic methods. Species identification was done by 16s rRNA sequence analysis. The functional probiotic potential of the two Lactobacillus species viz., Lactobacillus plantarum (Lact. plantarum) and Lactobacillus pentosus (Lact. pentosus) was assessed by different standard parameters. The strains were tolerant to pH 2 for 1h and resistant to methicillin, kanamycin, vancomycin and norfloxacin. Two (Lact. plantarum COORG-3 and Lact. pentosus COORG-8) out of eight isolates recorded the cell surface hydrophobicity to be 59.12 and 64.06%, respectively. All the strains showed tolerance to artificial duodenum juice (pH 2) for 3h, positive for bile salt hydrolase test and negative for haemolytic test. The neutralized cell-free supernatant of the strains Lact. pentosus COORG-4, Lact. plantarum COORG-1, Lact. plantarum COORG-7, Lact. pentosus COORG-8 and Lact. plantarum COORG-3 showed good antibiofilm activity. Lact. pentosus COORG-8 exhibited 74% activity against Pseudomonas aeruginosa-MTCC 7903 and Lact. plantarum COORG-7 showed 68% inhibition of biofilm against Klebsiella pneumonia MTCC 7407. Three (Lact. plantarum COORG-7, Lact. pentosus COORG-5 and Lact. pentosus COORG 8) out of eight isolates exhibited a good antimicrobial activity against Listeria monocytogenes and five isolates (Lact. pentosus COORG 2, Lact. plantarum COORG 1, Lact. plantarum COORG 4, Lact. pentosus COORG 3 and Lact. plantarum COORG 6) are active against Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa, Enterobacter aerogenes, Klebsiella pneumonia, Enterococcus faecalis. The study also evaluated the cholesterol lowering property of the Lactobacillus strains using hen egg yolk as the cholesterol source. The cholesterol in hen egg yolk was assimilated by 74.12 and 68.26% by Lact. plantarum COORG 4 and Lact. pentosus COORG 7, respectively. The results of the present study suggest that the Lactobacillus strains isolated and characterized from sorghum-based fermented product may be used as probiotic strains for therapeutic applications. Springer Science+Business Media New York, 2015 Fermented food nationallicence Lactobacillus plantarum nationallicence Lactobacillus pentosus nationallicence Probiotics nationallicence Biofilm nationallicence Poornachandra Rao K. Department of Studies in Microbiology, University of Mysore, Manasagangotri, 570 006, Mysore, Karnataka, India aut Chennappa G. Department of Studies in Microbiology, University of Mysore, Manasagangotri, 570 006, Mysore, Karnataka, India aut Suraj U. NRC on DNA Finger Printing, NBPGR, PUSA Campus, 110012, New Delhi, India aut Nagaraja H. Department of Studies in Microbiology, University of Mysore, Manasagangotri, 570 006, Mysore, Karnataka, India aut Charith Raj A. Laboratory of Plant Pathology, Faculty of Agriculture and Life Science, Hirosaki University, Bunkyo-cho 3, 036-8561, Hirosaki, Aomori Ken, Japan aut Sreenivasa M. Department of Studies in Microbiology, University of Mysore, Manasagangotri, 570 006, Mysore, Karnataka, India aut Probiotics and Antimicrobial Proteins Springer US; http://www.springer-ny.com 7/2(2015-06-01), 146-156 1867-1306 7:2<146 2015 7 12602 https://doi.org/10.1007/s12602-015-9186-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s12602-015-9186-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Poornachandra Rao K. Department of Studies in Microbiology, University of Mysore, Manasagangotri, 570 006, Mysore, Karnataka, India aut NATIONALLICENCE 700 1- Chennappa G. Department of Studies in Microbiology, University of Mysore, Manasagangotri, 570 006, Mysore, Karnataka, India aut NATIONALLICENCE 700 1- Suraj U. NRC on DNA Finger Printing, NBPGR, PUSA Campus, 110012, New Delhi, India aut NATIONALLICENCE 700 1- Nagaraja H. Department of Studies in Microbiology, University of Mysore, Manasagangotri, 570 006, Mysore, Karnataka, India aut NATIONALLICENCE 700 1- Charith Raj A. Laboratory of Plant Pathology, Faculty of Agriculture and Life Science, Hirosaki University, Bunkyo-cho 3, 036-8561, Hirosaki, Aomori Ken, Japan aut NATIONALLICENCE 700 1- Sreenivasa M. Department of Studies in Microbiology, University of Mysore, Manasagangotri, 570 006, Mysore, Karnataka, India aut NATIONALLICENCE 773 0- Probiotics and Antimicrobial Proteins Springer US; http://www.springer-ny.com 7/2(2015-06-01), 146-156 1867-1306 7:2<146 2015 7 12602