caa a22 4500 606242872 CHVBK 20210128101321.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s12551-014-0155-1 doi (NATIONALLICENCE)springer-10.1007/s12551-014-0155-1 Whitford Paul Department of Physics, Northeastern University Dana Research Center 123, 360 Huntington Ave, 02115, Boston, MA, USA aut The ribosome's energy landscape: Recent insights from computation [Elektronische Daten] [Paul Whitford] The ever-increasing capacity of computing resources has extended ribosome calculations from the study of small-scale fluctuations to large-scale barrier-crossing processes. As the field of computational/theoretical biophysics shifts focus to large-scale conformational transitions, there is a growing need for a systematic framework to interpret and analyze ribosome dynamics. To this end, energy landscape principles, largely developed for the study of biomolecular folding, have proven to be invaluable. These tools not only provide a foundation for describing simulations but can be used to reconcile experimental results, as well. In this review, I will discuss recent efforts to employ computational methods to reveal the characteristics of the ribosome's landscape and how these studies can help guide a new generation of experiments that more closely probe the underlying energetics. As a result of these investigations, general principles about ribosome function are beginning to emerge, including that: (1) small-scale fluctuations are the result of structure, rather than detailed energetics, (2) molecular flexibility leads to entropically favored rearrangements, and (3) tRNA dynamics may be accurately described as diffusive movement across an energy landscape. International Union for Pure and Applied Biophysics (IUPAB) and Springer-Verlag Berlin Heidelberg, 2015 Simulations nationallicence Molecular machine nationallicence tRNA nationallicence Elongation nationallicence Biophysical Reviews Springer Berlin Heidelberg 7/3(2015-09-01), 301-310 1867-2450 7:3<301 2015 7 12551 https://doi.org/10.1007/s12551-014-0155-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s12551-014-0155-1 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Whitford Paul Department of Physics, Northeastern University Dana Research Center 123, 360 Huntington Ave, 02115, Boston, MA, USA aut NATIONALLICENCE 773 0- Biophysical Reviews Springer Berlin Heidelberg 7/3(2015-09-01), 301-310 1867-2450 7:3<301 2015 7 12551